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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacteroides thetaiotaomicron Thiazole synthase (thiG) CSB-YP805201BDU
CSB-EP805201BDU
CSB-BP805201BDU
CSB-MP805201BDU
CSB-EP805201BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP805202BDU
CSB-EP805202BDU
CSB-BP805202BDU
CSB-MP805202BDU
CSB-EP805202BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Indole-3-glycerol phosphate synthase (trpC) CSB-YP805203BDU
CSB-EP805203BDU
CSB-BP805203BDU
CSB-MP805203BDU
CSB-EP805203BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP805204BDU
CSB-EP805204BDU
CSB-BP805204BDU
CSB-MP805204BDU
CSB-EP805204BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Histidinol-phosphate aminotransferase (hisC) CSB-YP805205BDU
CSB-EP805205BDU
CSB-BP805205BDU
CSB-MP805205BDU
CSB-EP805205BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S17 2 (rpsQ2) CSB-YP805206BDU
CSB-EP805206BDU
CSB-BP805206BDU
CSB-MP805206BDU
CSB-EP805206BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein arginine N-methyltransferase 1-A (prmt1-a) CSB-YP805207XBE
CSB-EP805207XBE
CSB-BP805207XBE
CSB-MP805207XBE
CSB-EP805207XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis T-box transcription factor TBX6 (tbx6), partial CSB-YP805208XBE
CSB-EP805208XBE
CSB-BP805208XBE
CSB-MP805208XBE
CSB-EP805208XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Malectin-B (mlec-b), partial CSB-YP805209XBE
CSB-EP805209XBE
CSB-BP805209XBE
CSB-MP805209XBE
CSB-EP805209XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Leucine-rich repeat protein SHOC-2 (shoc2), partial CSB-YP805210XBE
CSB-EP805210XBE
CSB-BP805210XBE
CSB-MP805210XBE
CSB-EP805210XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis V-type proton ATPase 116 kDa subunit a isoform 1 (atp6v0a1), partial CSB-YP805211XBE
CSB-EP805211XBE
CSB-BP805211XBE
CSB-MP805211XBE
CSB-EP805211XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DDB1- and CUL4-associated factor 10 (dcaf10) CSB-YP805212XBE
CSB-EP805212XBE
CSB-BP805212XBE
CSB-MP805212XBE
CSB-EP805212XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein XRP2 (rp2) CSB-YP805213XBE
CSB-EP805213XBE
CSB-BP805213XBE
CSB-MP805213XBE
CSB-EP805213XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein atonal homolog 7 (atoh7) CSB-YP805214DIL
CSB-EP805214DIL
CSB-BP805214DIL
CSB-MP805214DIL
CSB-EP805214DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis niloticus Nascent polypeptide-associated complex subunit alpha (naca) CSB-YP805215OET
CSB-EP805215OET
CSB-BP805215OET
CSB-MP805215OET
CSB-EP805215OET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Transcription factor Sox-17-beta.1 (sox17b.1) CSB-YP805216XBF
CSB-EP805216XBF
CSB-BP805216XBF
CSB-MP805216XBF
CSB-EP805216XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio RuvB-like 1 (ruvbl1) CSB-YP805217DIL
CSB-EP805217DIL
CSB-BP805217DIL
CSB-MP805217DIL
CSB-EP805217DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atheris squamigera Serine/threonine-protein kinase mos (MOS) CSB-YP805218AQL
CSB-EP805218AQL
CSB-BP805218AQL
CSB-MP805218AQL
CSB-EP805218AQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis F-box only protein 43 (fbxo43), partial CSB-YP805219XBE
CSB-EP805219XBE
CSB-BP805219XBE
CSB-MP805219XBE
CSB-EP805219XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss C-type natriuretic peptide 2 (cnp-1-2) CSB-YP805220OEI
CSB-EP805220OEI
CSB-BP805220OEI
CSB-MP805220OEI
CSB-EP805220OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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