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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP805635CMN
CSB-EP805635CMN
CSB-BP805635CMN
CSB-MP805635CMN
CSB-EP805635CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soil-borne wheat mosaic virus Replicase large subunit (rep), partial CSB-YP805636SIK
CSB-EP805636SIK
CSB-BP805636SIK
CSB-MP805636SIK
CSB-EP805636SIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dephospho-CoA kinase (coaE) CSB-YP805637CMN
CSB-EP805637CMN
CSB-BP805637CMN
CSB-MP805637CMN
CSB-EP805637CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 4 (lepA), partial CSB-YP805638CMN
CSB-EP805638CMN
CSB-BP805638CMN
CSB-MP805638CMN
CSB-EP805638CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal protein L11 methyltransferase (prmA) CSB-YP805639CMN
CSB-EP805639CMN
CSB-BP805639CMN
CSB-MP805639CMN
CSB-EP805639CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP805640CMN
CSB-EP805640CMN
CSB-BP805640CMN
CSB-MP805640CMN
CSB-EP805640CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A 1 (truA1) CSB-YP805641CMN
CSB-EP805641CMN
CSB-BP805641CMN
CSB-MP805641CMN
CSB-EP805641CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydroxyethylthiazole kinase (thiM) CSB-YP805642CMN
CSB-EP805642CMN
CSB-BP805642CMN
CSB-MP805642CMN
CSB-EP805642CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein D129L (Ba71V-103) CSB-YP805643AEJ
CSB-EP805643AEJ
CSB-BP805643AEJ
CSB-MP805643AEJ
CSB-EP805643AEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP805644CMN
CSB-EP805644CMN
CSB-BP805644CMN
CSB-MP805644CMN
CSB-EP805644CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP805645CMN
CSB-EP805645CMN
CSB-BP805645CMN
CSB-MP805645CMN
CSB-EP805645CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein htpG (htpG), partial CSB-YP805646CMN
CSB-EP805646CMN
CSB-BP805646CMN
CSB-MP805646CMN
CSB-EP805646CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 40 Major core protein (PVII) CSB-YP805647HIR
CSB-EP805647HIR
CSB-BP805647HIR
CSB-MP805647HIR
CSB-EP805647HIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Reston ebolavirus Pre-small/secreted glycoprotein (GP) CSB-YP805648RCAZ
CSB-EP805648RCAZ
CSB-BP805648RCAZ
CSB-MP805648RCAZ
CSB-EP805648RCAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytidylate kinase (cmk) CSB-YP805649CMN
CSB-EP805649CMN
CSB-BP805649CMN
CSB-MP805649CMN
CSB-EP805649CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA dimethylallyltransferase (miaA) CSB-YP805650CMN
CSB-EP805650CMN
CSB-BP805650CMN
CSB-MP805650CMN
CSB-EP805650CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome maturation factor RimP (rimP) CSB-YP805651CMN
CSB-EP805651CMN
CSB-BP805651CMN
CSB-MP805651CMN
CSB-EP805651CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridylate kinase (pyrH) CSB-YP805652CMN
CSB-EP805652CMN
CSB-BP805652CMN
CSB-MP805652CMN
CSB-EP805652CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP805653CMN
CSB-EP805653CMN
CSB-BP805653CMN
CSB-MP805653CMN
CSB-EP805653CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopantetheine adenylyltransferase (coaD) CSB-YP805654CMN
CSB-EP805654CMN
CSB-BP805654CMN
CSB-MP805654CMN
CSB-EP805654CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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