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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 30S ribosomal protein S15 (rpsO) CSB-YP805695BMZ
CSB-EP805695BMZ
CSB-BP805695BMZ
CSB-MP805695BMZ
CSB-EP805695BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Valine--tRNA ligase (valS), partial CSB-YP805696BMZ
CSB-EP805696BMZ
CSB-BP805696BMZ
CSB-MP805696BMZ
CSB-EP805696BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 3-oxoacyl-[acyl-carrier-protein] reductase FabG (fabG) CSB-YP805697BMZ
CSB-EP805697BMZ
CSB-BP805697BMZ
CSB-MP805697BMZ
CSB-EP805697BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar basal-body rod protein flgC (flgC) CSB-YP805698BMZ
CSB-EP805698BMZ
CSB-BP805698BMZ
CSB-MP805698BMZ
CSB-EP805698BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Zinc import ATP-binding protein ZnuC (znuC) CSB-YP805699BMZ
CSB-EP805699BMZ
CSB-BP805699BMZ
CSB-MP805699BMZ
CSB-EP805699BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae UPF0135 protein bbp_279 (bbp_279) CSB-YP805700BMZ
CSB-EP805700BMZ
CSB-BP805700BMZ
CSB-MP805700BMZ
CSB-EP805700BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Probable intracellular septation protein A (bbp_255), partial CSB-YP805701BMZ1
CSB-EP805701BMZ1
CSB-BP805701BMZ1
CSB-MP805701BMZ1
CSB-EP805701BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (fabI) CSB-YP805702BMZ
CSB-EP805702BMZ
CSB-BP805702BMZ
CSB-MP805702BMZ
CSB-EP805702BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Protein grpE 1 (grpE1) CSB-YP805703BMZ
CSB-EP805703BMZ
CSB-BP805703BMZ
CSB-MP805703BMZ
CSB-EP805703BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA polymerase III subunit alpha (dnaE), partial CSB-YP805704BMZ
CSB-EP805704BMZ
CSB-BP805704BMZ
CSB-MP805704BMZ
CSB-EP805704BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP805705BMZ
CSB-EP805705BMZ
CSB-BP805705BMZ
CSB-MP805705BMZ
CSB-EP805705BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Peptidoglycan synthase FtsI (ftsI), partial CSB-YP805706BMZ
CSB-EP805706BMZ
CSB-BP805706BMZ
CSB-MP805706BMZ
CSB-EP805706BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Pyruvate dehydrogenase E1 component (aceE), partial CSB-YP805707BMZ
CSB-EP805707BMZ
CSB-BP805707BMZ
CSB-MP805707BMZ
CSB-EP805707BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Protein grpE 2 (grpE2) CSB-YP805708BMZ
CSB-EP805708BMZ
CSB-BP805708BMZ
CSB-MP805708BMZ
CSB-EP805708BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae NADH-quinone oxidoreductase subunit M (nuoM), partial CSB-YP805709BMZ
CSB-EP805709BMZ
CSB-BP805709BMZ
CSB-MP805709BMZ
CSB-EP805709BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae NADH-quinone oxidoreductase subunit G (nuoG), partial CSB-YP805710BMZ
CSB-EP805710BMZ
CSB-BP805710BMZ
CSB-MP805710BMZ
CSB-EP805710BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Isoleucine--tRNA ligase (ileS), partial CSB-YP805711BMZ
CSB-EP805711BMZ
CSB-BP805711BMZ
CSB-MP805711BMZ
CSB-EP805711BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L35 (rpmI) CSB-YP805712BMZ
CSB-EP805712BMZ
CSB-BP805712BMZ
CSB-MP805712BMZ
CSB-EP805712BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Electron transport complex protein RnfB (rnfB) CSB-YP805713BMZ
CSB-EP805713BMZ
CSB-BP805713BMZ
CSB-MP805713BMZ
CSB-EP805713BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Methionine--tRNA ligase (metG), partial CSB-YP805714BMZ
CSB-EP805714BMZ
CSB-BP805714BMZ
CSB-MP805714BMZ
CSB-EP805714BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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