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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis FGFR1 oncogene partner 2 homolog (fgfr1op2) CSB-YP805835XBE
CSB-EP805835XBE
CSB-BP805835XBE
CSB-MP805835XBE
CSB-EP805835XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Pancreatic progenitor cell differentiation and proliferation factor A (ppdpf-a) CSB-YP805836XBE
CSB-EP805836XBE
CSB-BP805836XBE
CSB-MP805836XBE
CSB-EP805836XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Estradiol 17-beta-dehydrogenase 12-B (hsd17b12-b), partial CSB-YP805837XBE1
CSB-EP805837XBE1
CSB-BP805837XBE1
CSB-MP805837XBE1
CSB-EP805837XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lampetra fluviatilis Keratin, type 1 cytoskeletal 11 CSB-YP805838LNM
CSB-EP805838LNM
CSB-BP805838LNM
CSB-MP805838LNM
CSB-EP805838LNM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Kinetochore protein Nuf2-A (nuf2-a) CSB-YP805839XBE
CSB-EP805839XBE
CSB-BP805839XBE
CSB-MP805839XBE
CSB-EP805839XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF128 (rnf128), partial CSB-YP805840XBE1
CSB-EP805840XBE1
CSB-BP805840XBE1
CSB-MP805840XBE1
CSB-EP805840XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein Hox-A3a (hoxa3a) CSB-YP805841DIL
CSB-EP805841DIL
CSB-BP805841DIL
CSB-MP805841DIL
CSB-EP805841DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes Mast/stem cell growth factor receptor Kit (kit), partial CSB-YP805842TKY
CSB-EP805842TKY
CSB-BP805842TKY
CSB-MP805842TKY
CSB-EP805842TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Seriola quinqueradiata SH3 domain-binding protein 4 (sh3bp4), partial CSB-YP805843SFI
CSB-EP805843SFI
CSB-BP805843SFI
CSB-MP805843SFI
CSB-EP805843SFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus gorbuscha Calcitonin gene-related peptide type 1 receptor (calcrl), partial CSB-YP805844OBF1
CSB-EP805844OBF1
CSB-BP805844OBF1
CSB-MP805844OBF1
CSB-EP805844OBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Muscle, skeletal receptor tyrosine protein kinase (MUSK), partial CSB-YP805845CH
CSB-EP805845CH
CSB-BP805845CH
CSB-MP805845CH
CSB-EP805845CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Protease inhibitor C CSB-YP805846BPN
CSB-EP805846BPN
CSB-BP805846BPN
CSB-MP805846BPN
CSB-EP805846BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Phospholipase A2, beta bungarotoxin A2 chain CSB-YP805847BPN
CSB-EP805847BPN
CSB-BP805847BPN
CSB-MP805847BPN
CSB-EP805847BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus candidus Kappa 1b-bungarotoxin CSB-YP805848BPN
CSB-EP805848BPN
CSB-BP805848BPN
CSB-MP805848BPN
CSB-EP805848BPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease 5 CSB-YP805849VDN
CSB-EP805849VDN
CSB-BP805849VDN
CSB-MP805849VDN
CSB-EP805849VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeodomain-only protein (hopx) CSB-YP805850DIL
CSB-EP805850DIL
CSB-BP805850DIL
CSB-MP805850DIL
CSB-EP805850DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Green-sensitive opsin-2 (opn1mw2), partial CSB-YP805851DIL1
CSB-EP805851DIL1
CSB-BP805851DIL1
CSB-MP805851DIL1
CSB-EP805851DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen leader protein (EBNA-LP), partial CSB-YP805852EFA
CSB-EP805852EFA
CSB-BP805852EFA
CSB-MP805852EFA
CSB-EP805852EFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicular stomatitis Indiana virus Matrix protein (M) CSB-YP805853VBD
CSB-EP805853VBD
CSB-BP805853VBD
CSB-MP805853VBD
CSB-EP805853VBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vesicular stomatitis Indiana virus Phosphoprotein (P) CSB-YP805854VBF
CSB-EP805854VBF
CSB-BP805854VBF
CSB-MP805854VBF
CSB-EP805854VBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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