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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP805655CMN
CSB-EP805655CMN
CSB-BP805655CMN
CSB-MP805655CMN
CSB-EP805655CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional repressor NrdR (nrdR) CSB-YP805656CMN
CSB-EP805656CMN
CSB-BP805656CMN
CSB-MP805656CMN
CSB-EP805656CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Uncharacterized protein KP86R/DP86L (BA71V-001) CSB-YP805657AEJ
CSB-EP805657AEJ
CSB-BP805657AEJ
CSB-MP805657AEJ
CSB-EP805657AEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridine kinase (udk) CSB-YP805658CMN
CSB-EP805658CMN
CSB-BP805658CMN
CSB-MP805658CMN
CSB-EP805658CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HPr kinase/phosphorylase (hprK) CSB-YP805659CMN
CSB-EP805659CMN
CSB-BP805659CMN
CSB-MP805659CMN
CSB-EP805659CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0303 protein CTC_00911 (CTC_00911) CSB-YP805660CMN
CSB-EP805660CMN
CSB-BP805660CMN
CSB-MP805660CMN
CSB-EP805660CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Protein Vpx (vpx) CSB-YP805661HME
CSB-EP805661HME
CSB-BP805661HME
CSB-MP805661HME
CSB-EP805661HME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 505-4R (BA71R-027), partial CSB-YP805662AEJ
CSB-EP805662AEJ
CSB-BP805662AEJ
CSB-MP805662AEJ
CSB-EP805662AEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphomethylpyrimidine synthase (thiC) CSB-YP805663CMN
CSB-EP805663CMN
CSB-BP805663CMN
CSB-MP805663CMN
CSB-EP805663CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobyrinic acid A,C-diamide synthase (cobB) CSB-YP805664CMN
CSB-EP805664CMN
CSB-BP805664CMN
CSB-MP805664CMN
CSB-EP805664CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP805665CMN
CSB-EP805665CMN
CSB-BP805665CMN
CSB-MP805665CMN
CSB-EP805665CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Reston ebolavirus Envelope glycoprotein (GP), partial CSB-YP805666RCAZ
CSB-EP805666RCAZ
CSB-BP805666RCAZ
CSB-MP805666RCAZ
CSB-EP805666RCAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP805667CMN
CSB-EP805667CMN
CSB-BP805667CMN
CSB-MP805667CMN
CSB-EP805667CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative competence-damage inducible protein (cinA) CSB-YP805668CMN
CSB-EP805668CMN
CSB-BP805668CMN
CSB-MP805668CMN
CSB-EP805668CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidine kinase (tdk) CSB-YP805669CMN
CSB-EP805669CMN
CSB-BP805669CMN
CSB-MP805669CMN
CSB-EP805669CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit hemorrhagic disease virus Protein VP2 (ORF2) CSB-YP805670RCAW
CSB-EP805670RCAW
CSB-BP805670RCAW
CSB-MP805670RCAW
CSB-EP805670RCAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP805671CMN
CSB-EP805671CMN
CSB-BP805671CMN
CSB-MP805671CMN
CSB-EP805671CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD-dependent protein deacetylase (cobB) CSB-YP805672CMN
CSB-EP805672CMN
CSB-BP805672CMN
CSB-MP805672CMN
CSB-EP805672CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidyl-tRNA hydrolase (pth) CSB-YP805673CMN
CSB-EP805673CMN
CSB-BP805673CMN
CSB-MP805673CMN
CSB-EP805673CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP805674CMN
CSB-EP805674CMN
CSB-BP805674CMN
CSB-MP805674CMN
CSB-EP805674CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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