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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacteroides thetaiotaomicron Release factor glutamine methyltransferase (prmC) CSB-YP805795BDU
CSB-EP805795BDU
CSB-BP805795BDU
CSB-MP805795BDU
CSB-EP805795BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L34 (rpmH) CSB-YP805796BDU
CSB-EP805796BDU
CSB-BP805796BDU
CSB-MP805796BDU
CSB-EP805796BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Protein MraZ (mraZ) CSB-YP805797BDU
CSB-EP805797BDU
CSB-BP805797BDU
CSB-MP805797BDU
CSB-EP805797BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP805798BDU
CSB-EP805798BDU
CSB-BP805798BDU
CSB-MP805798BDU
CSB-EP805798BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Shikimate kinase (aroK) CSB-YP805799BDU
CSB-EP805799BDU
CSB-BP805799BDU
CSB-MP805799BDU
CSB-EP805799BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP805800BDU
CSB-EP805800BDU
CSB-BP805800BDU
CSB-MP805800BDU
CSB-EP805800BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Undecaprenyl-diphosphatase (uppP), partial CSB-YP805801BDU1
CSB-EP805801BDU1
CSB-BP805801BDU1
CSB-MP805801BDU1
CSB-EP805801BDU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Phosphopantetheine adenylyltransferase (coaD) CSB-YP805802BDU
CSB-EP805802BDU
CSB-BP805802BDU
CSB-MP805802BDU
CSB-EP805802BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Elongation factor Tu (tuf) CSB-YP805803BDU
CSB-EP805803BDU
CSB-BP805803BDU
CSB-MP805803BDU
CSB-EP805803BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L3 (rplC) CSB-YP805804BDU
CSB-EP805804BDU
CSB-BP805804BDU
CSB-MP805804BDU
CSB-EP805804BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L14 (rplN) CSB-YP805805BDU
CSB-EP805805BDU
CSB-BP805805BDU
CSB-MP805805BDU
CSB-EP805805BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L18 (rplR) CSB-YP805806BDU
CSB-EP805806BDU
CSB-BP805806BDU
CSB-MP805806BDU
CSB-EP805806BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP805807BDU
CSB-EP805807BDU
CSB-BP805807BDU
CSB-MP805807BDU
CSB-EP805807BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Glycine cleavage system H protein (gcvH) CSB-YP805808BDU
CSB-EP805808BDU
CSB-BP805808BDU
CSB-MP805808BDU
CSB-EP805808BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Putative ribosome biogenesis GTPase RsgA 2 (rsgA2) CSB-YP805809BDU
CSB-EP805809BDU
CSB-BP805809BDU
CSB-MP805809BDU
CSB-EP805809BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 30S ribosomal protein S18 (rpsR) CSB-YP805810BDU
CSB-EP805810BDU
CSB-BP805810BDU
CSB-MP805810BDU
CSB-EP805810BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Uncharacterized transporter BT_2092 (BT_2092), partial CSB-YP805811BDU
CSB-EP805811BDU
CSB-BP805811BDU
CSB-MP805811BDU
CSB-EP805811BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Cytidylate kinase (cmk) CSB-YP805812BDU
CSB-EP805812BDU
CSB-BP805812BDU
CSB-MP805812BDU
CSB-EP805812BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP805813BDU
CSB-EP805813BDU
CSB-BP805813BDU
CSB-MP805813BDU
CSB-EP805813BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 10 kDa chaperonin (groS) CSB-YP805814BDU
CSB-EP805814BDU
CSB-BP805814BDU
CSB-MP805814BDU
CSB-EP805814BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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