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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dinoroseobacter shibae GTPase Era (era) CSB-YP007759DIB
CSB-EP007759DIB
CSB-BP007759DIB
CSB-MP007759DIB
CSB-EP007759DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Peptide chain release factor 1 (prfA) CSB-YP015197DIB
CSB-EP015197DIB
CSB-BP015197DIB
CSB-MP015197DIB
CSB-EP015197DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Acyl carrier protein (acpP) CSB-YP015636DIB
CSB-EP015636DIB
CSB-BP015636DIB
CSB-MP015636DIB
CSB-EP015636DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Manganese-dependent ADP-ribose/CDP-alcohol diphosphatase CSB-YP003349BO
CSB-EP003349BO
CSB-BP003349BO
CSB-MP003349BO
CSB-EP003349BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Hsp90 co-chaperone Cdc37-like 1 (cdc37l1) CSB-YP005006DIL
CSB-EP005006DIL
CSB-BP005006DIL
CSB-MP005006DIL
CSB-EP005006DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Translation initiation factor IF-2 (infB), partial CSB-YP015164BQD
CSB-EP015164BQD
CSB-BP015164BQD
CSB-MP015164BQD
CSB-EP015164BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Porphobilinogen deaminase (hemC) CSB-YP010524BQD
CSB-EP010524BQD
CSB-BP010524BQD
CSB-MP010524BQD
CSB-EP010524BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Glucose-6-phosphate isomerase (pgi) CSB-YP009717BQD
CSB-EP009717BQD
CSB-BP009717BQD
CSB-MP009717BQD
CSB-EP009717BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens ATP synthase subunit alpha (atpA), partial CSB-YP002344BQD
CSB-EP002344BQD
CSB-BP002344BQD
CSB-MP002344BQD
CSB-EP002344BQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus tRNA dimethylallyltransferase (miaA) CSB-YP024529CWA
CSB-EP024529CWA
CSB-BP024529CWA
CSB-MP024529CWA
CSB-EP024529CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus ATP synthase subunit b (atpF), partial CSB-YP002358CWA1
CSB-EP002358CWA1
CSB-BP002358CWA1
CSB-MP002358CWA1
CSB-EP002358CWA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus ATP synthase subunit a (atpB), partial CSB-YP015070CWA1
CSB-EP015070CWA1
CSB-BP015070CWA1
CSB-MP015070CWA1
CSB-EP015070CWA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Ferrochelatase (hemH) CSB-YP008579CWA
CSB-EP008579CWA
CSB-BP008579CWA
CSB-MP008579CWA
CSB-EP008579CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Acyl carrier protein (acpP) CSB-YP015636CWA
CSB-EP015636CWA
CSB-BP015636CWA
CSB-MP015636CWA
CSB-EP015636CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Beta-galactosidase (lacZ), partial CSB-YP009476EJD
CSB-EP009476EJD
CSB-BP009476EJD
CSB-MP009476EJD
CSB-EP009476EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824EJD1
CSB-EP005824EJD1
CSB-BP005824EJD1
CSB-MP005824EJD1
CSB-EP005824EJD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Nucleoside diphosphate kinase (ndk) CSB-YP015889EJD
CSB-EP015889EJD
CSB-BP015889EJD
CSB-MP015889EJD
CSB-EP015889EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 N-acetylneuraminate lyase (nanA) CSB-YP015994EJD
CSB-EP015994EJD
CSB-BP015994EJD
CSB-MP015994EJD
CSB-EP015994EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Glucose-6-phosphate isomerase (pgi) CSB-YP009717EJD
CSB-EP009717EJD
CSB-BP009717EJD
CSB-MP009717EJD
CSB-EP009717EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Glycine cleavage system H protein (gcvH) CSB-YP009335EJF
CSB-EP009335EJF
CSB-BP009335EJF
CSB-MP009335EJF
CSB-EP009335EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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