Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella canis Ferrochelatase (hemH) CSB-YP008579BPH
CSB-EP008579BPH
CSB-BP008579BPH
CSB-MP008579BPH
CSB-EP008579BPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415STE
CSB-EP015415STE
CSB-BP015415STE
CSB-MP015415STE
CSB-EP015415STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae ATP synthase subunit beta (atpD) CSB-YP002350STE
CSB-EP002350STE
CSB-BP002350STE
CSB-MP002350STE
CSB-EP002350STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae L-threonine 3-dehydrogenase (tdh) CSB-YP023350STE
CSB-EP023350STE
CSB-BP023350STE
CSB-MP023350STE
CSB-EP023350STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Peptide deformylase (def) CSB-YP017707STE
CSB-EP017707STE
CSB-BP017707STE
CSB-MP017707STE
CSB-EP017707STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae NAD-dependent malic enzyme (maeA), partial CSB-YP013633STE
CSB-EP013633STE
CSB-BP013633STE
CSB-MP013633STE
CSB-EP013633STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella arizonae Glycine cleavage system H protein (gcvH) CSB-YP009335STE
CSB-EP009335STE
CSB-BP009335STE
CSB-MP009335STE
CSB-EP009335STE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Galactokinase (galK) CSB-YP009198STF
CSB-EP009198STF
CSB-BP009198STF
CSB-MP009198STF
CSB-EP009198STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B ATP synthase subunit delta (atpH) CSB-YP002355STF
CSB-EP002355STF
CSB-BP002355STF
CSB-MP002355STF
CSB-EP002355STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B NAD-dependent malic enzyme (maeA), partial CSB-YP013633STF
CSB-EP013633STF
CSB-BP013633STF
CSB-MP013633STF
CSB-EP013633STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Agmatinase (speB) CSB-YP001447STF
CSB-EP001447STF
CSB-BP001447STF
CSB-MP001447STF
CSB-EP001447STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Acyl carrier protein (acpP) CSB-YP015636STF
CSB-EP015636STF
CSB-BP015636STF
CSB-MP015636STF
CSB-EP015636STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome-recycling factor (frr) CSB-YP014941CXH
CSB-EP014941CXH
CSB-BP014941CXH
CSB-MP014941CXH
CSB-EP014941CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917CXH
CSB-EP022917CXH
CSB-BP022917CXH
CSB-MP022917CXH
CSB-EP022917CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Triosephosphate isomerase (tpiA) CSB-YP024102CXH
CSB-EP024102CXH
CSB-BP024102CXH
CSB-MP024102CXH
CSB-EP024102CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP synthase subunit beta (atpD) CSB-YP002350CXH
CSB-EP002350CXH
CSB-BP002350CXH
CSB-MP002350CXH
CSB-EP002350CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Lipoyl synthase (lipA) CSB-YP012927CXH
CSB-EP012927CXH
CSB-BP012927CXH
CSB-MP012927CXH
CSB-EP012927CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii ATP synthase subunit alpha (atpA), partial CSB-YP002344ATX
CSB-EP002344ATX
CSB-BP002344ATX
CSB-MP002344ATX
CSB-EP002344ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Glycine cleavage system H protein (gcvH) CSB-YP009335YAM
CSB-EP009335YAM
CSB-BP009335YAM
CSB-MP009335YAM
CSB-EP009335YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua ATP synthase subunit beta (atpD) CSB-YP002350YAM
CSB-EP002350YAM
CSB-BP002350YAM
CSB-MP002350YAM
CSB-EP002350YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>