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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP806109CMN
CSB-EP806109CMN
CSB-BP806109CMN
CSB-MP806109CMN
CSB-EP806109CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0042 nucleotide-binding protein CTC_02495 (CTC_02495) CSB-YP806110CMN
CSB-EP806110CMN
CSB-BP806110CMN
CSB-MP806110CMN
CSB-EP806110CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase (A49L) CSB-YP806111VAR
CSB-EP806111VAR
CSB-BP806111VAR
CSB-MP806111VAR
CSB-EP806111VAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP806112CMN
CSB-EP806112CMN
CSB-BP806112CMN
CSB-MP806112CMN
CSB-EP806112CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP806113CMN
CSB-EP806113CMN
CSB-BP806113CMN
CSB-MP806113CMN
CSB-EP806113CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein recX (recX) CSB-YP806114CMN
CSB-EP806114CMN
CSB-BP806114CMN
CSB-MP806114CMN
CSB-EP806114CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP806115CMN
CSB-EP806115CMN
CSB-BP806115CMN
CSB-MP806115CMN
CSB-EP806115CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP806116CMN
CSB-EP806116CMN
CSB-BP806116CMN
CSB-MP806116CMN
CSB-EP806116CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yellow fever virus Genome polyprotein, partial CSB-YP806117YAAL
CSB-EP806117YAAL
CSB-BP806117YAAL
CSB-MP806117YAAL
CSB-EP806117YAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaK (dnaK), partial CSB-YP806118CMN
CSB-EP806118CMN
CSB-BP806118CMN
CSB-MP806118CMN
CSB-EP806118CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional purine biosynthesis protein PurH (purH) CSB-YP806119CMN
CSB-EP806119CMN
CSB-BP806119CMN
CSB-MP806119CMN
CSB-EP806119CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0261 protein CTC_01794 (CTC_01794) CSB-YP806120CMN
CSB-EP806120CMN
CSB-BP806120CMN
CSB-MP806120CMN
CSB-EP806120CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Major structural protein p17 (Ba71V-107), partial CSB-YP806121AEJ1
CSB-EP806121AEJ1
CSB-BP806121AEJ1
CSB-MP806121AEJ1
CSB-EP806121AEJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variola virus Truncated protein A35 homolog (A38R) CSB-YP806122VAR
CSB-EP806122VAR
CSB-BP806122VAR
CSB-MP806122VAR
CSB-EP806122VAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoprotein signal peptidase (lspA), partial CSB-YP806123CMN1
CSB-EP806123CMN1
CSB-BP806123CMN1
CSB-MP806123CMN1
CSB-EP806123CMN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine repressor (argR) CSB-YP806124CMN
CSB-EP806124CMN
CSB-BP806124CMN
CSB-MP806124CMN
CSB-EP806124CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphopentomutase (deoB) CSB-YP806125CMN
CSB-EP806125CMN
CSB-BP806125CMN
CSB-MP806125CMN
CSB-EP806125CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP806126IMU
CSB-EP806126IMU
CSB-BP806126IMU
CSB-MP806126IMU
CSB-EP806126IMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP806127CMN
CSB-EP806127CMN
CSB-BP806127CMN
CSB-MP806127CMN
CSB-EP806127CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LexA repressor (lexA) CSB-YP806128CMN
CSB-EP806128CMN
CSB-BP806128CMN
CSB-MP806128CMN
CSB-EP806128CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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