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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized protein bbp_236 (bbp_236) CSB-YP807170BMZ
CSB-EP807170BMZ
CSB-BP807170BMZ
CSB-MP807170BMZ
CSB-EP807170BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar biosynthetic protein flhB (flhB), partial CSB-YP807171BMZ1
CSB-EP807171BMZ1
CSB-BP807171BMZ1
CSB-MP807171BMZ1
CSB-EP807171BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Cell division protein FtsL (ftsL), partial CSB-YP807172BMZ
CSB-EP807172BMZ
CSB-BP807172BMZ
CSB-MP807172BMZ
CSB-EP807172BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Cell division protein FtsA (ftsA) CSB-YP807173BMZ
CSB-EP807173BMZ
CSB-BP807173BMZ
CSB-MP807173BMZ
CSB-EP807173BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Protein translocase subunit SecA (secA), partial CSB-YP807174BMZ
CSB-EP807174BMZ
CSB-BP807174BMZ
CSB-MP807174BMZ
CSB-EP807174BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA gyrase subunit A (gyrA), partial CSB-YP807175BMZ
CSB-EP807175BMZ
CSB-BP807175BMZ
CSB-MP807175BMZ
CSB-EP807175BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Release factor glutamine methyltransferase (prmC) CSB-YP807176BMZ
CSB-EP807176BMZ
CSB-BP807176BMZ
CSB-MP807176BMZ
CSB-EP807176BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae NADH-quinone oxidoreductase subunit J (nuoJ), partial CSB-YP807177BMZ1
CSB-EP807177BMZ1
CSB-BP807177BMZ1
CSB-MP807177BMZ1
CSB-EP807177BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP807178BMZ1
CSB-EP807178BMZ1
CSB-BP807178BMZ1
CSB-MP807178BMZ1
CSB-EP807178BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP807179BMZ
CSB-EP807179BMZ
CSB-BP807179BMZ
CSB-MP807179BMZ
CSB-EP807179BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Tyrosine--tRNA ligase (tyrS) CSB-YP807180BMZ
CSB-EP807180BMZ
CSB-BP807180BMZ
CSB-MP807180BMZ
CSB-EP807180BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae UPF0115 protein bbp_092 (bbp_092) CSB-YP807181BMZ
CSB-EP807181BMZ
CSB-BP807181BMZ
CSB-MP807181BMZ
CSB-EP807181BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 3-oxoacyl-[acyl-carrier-protein] synthase 1 (fabB) CSB-YP807182BMZ
CSB-EP807182BMZ
CSB-BP807182BMZ
CSB-MP807182BMZ
CSB-EP807182BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Flagellar motor switch protein FliN (fliN) CSB-YP807183BMZ
CSB-EP807183BMZ
CSB-BP807183BMZ
CSB-MP807183BMZ
CSB-EP807183BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Phosphocarrier protein HPr (ptsH) CSB-YP807184BMZ
CSB-EP807184BMZ
CSB-BP807184BMZ
CSB-MP807184BMZ
CSB-EP807184BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L11 (rplK) CSB-YP807185BMZ
CSB-EP807185BMZ
CSB-BP807185BMZ
CSB-MP807185BMZ
CSB-EP807185BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae DNA-binding protein HU (hup) CSB-YP807186BMZ
CSB-EP807186BMZ
CSB-BP807186BMZ
CSB-MP807186BMZ
CSB-EP807186BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Ribosomal RNA small subunit methyltransferase D (rsmD) CSB-YP807187BMZ
CSB-EP807187BMZ
CSB-BP807187BMZ
CSB-MP807187BMZ
CSB-EP807187BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L34 (rpmH) CSB-YP807188BMZ
CSB-EP807188BMZ
CSB-BP807188BMZ
CSB-MP807188BMZ
CSB-EP807188BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae ATP synthase gamma chain (atpG) CSB-YP807189BMZ
CSB-EP807189BMZ
CSB-BP807189BMZ
CSB-MP807189BMZ
CSB-EP807189BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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