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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP807790VFI
CSB-EP807790VFI
CSB-BP807790VFI
CSB-MP807790VFI
CSB-EP807790VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Bifunctional pantoate ligase/cytidylate kinase (panC/cmk), partial CSB-YP807791TDQ
CSB-EP807791TDQ
CSB-BP807791TDQ
CSB-MP807791TDQ
CSB-EP807791TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP807792TDQ
CSB-EP807792TDQ
CSB-BP807792TDQ
CSB-MP807792TDQ
CSB-EP807792TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (chlL) CSB-YP807793TDQ
CSB-EP807793TDQ
CSB-BP807793TDQ
CSB-MP807793TDQ
CSB-EP807793TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Putative Holliday junction resolvase (tll2329) CSB-YP807794TDQ
CSB-EP807794TDQ
CSB-BP807794TDQ
CSB-MP807794TDQ
CSB-EP807794TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus DNA repair protein recO (recO) CSB-YP807795TDQ
CSB-EP807795TDQ
CSB-BP807795TDQ
CSB-MP807795TDQ
CSB-EP807795TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Chaperone protein dnaK3 (dnaK3), partial CSB-YP807796TDQ
CSB-EP807796TDQ
CSB-BP807796TDQ
CSB-MP807796TDQ
CSB-EP807796TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus LL-diaminopimelate aminotransferase (dapL) CSB-YP807797TDQ
CSB-EP807797TDQ
CSB-BP807797TDQ
CSB-MP807797TDQ
CSB-EP807797TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Photosystem II reaction center protein M (psbM), partial CSB-YP807798TDQ
CSB-EP807798TDQ
CSB-BP807798TDQ
CSB-MP807798TDQ
CSB-EP807798TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Dihydrodipicolinate reductase (dapB) CSB-YP807799TDQ
CSB-EP807799TDQ
CSB-BP807799TDQ
CSB-MP807799TDQ
CSB-EP807799TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Inorganic pyrophosphatase (ppa) CSB-YP807800TDQ
CSB-EP807800TDQ
CSB-BP807800TDQ
CSB-MP807800TDQ
CSB-EP807800TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Orotate phosphoribosyltransferase (pyrE) CSB-YP807801TDQ
CSB-EP807801TDQ
CSB-BP807801TDQ
CSB-MP807801TDQ
CSB-EP807801TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Phosphoglucosamine mutase (glmM) CSB-YP807802TDQ
CSB-EP807802TDQ
CSB-BP807802TDQ
CSB-MP807802TDQ
CSB-EP807802TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Elongation factor G (fusA), partial CSB-YP807803TDQ
CSB-EP807803TDQ
CSB-BP807803TDQ
CSB-MP807803TDQ
CSB-EP807803TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2 (acsF2) CSB-YP807804TDQ
CSB-EP807804TDQ
CSB-BP807804TDQ
CSB-MP807804TDQ
CSB-EP807804TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP807805TDQ
CSB-EP807805TDQ
CSB-BP807805TDQ
CSB-MP807805TDQ
CSB-EP807805TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Peptide deformylase (def) CSB-YP807806TDQ
CSB-EP807806TDQ
CSB-BP807806TDQ
CSB-MP807806TDQ
CSB-EP807806TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Translation initiation factor IF-3 (infC) CSB-YP807807TDQ
CSB-EP807807TDQ
CSB-BP807807TDQ
CSB-MP807807TDQ
CSB-EP807807TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Probable rRNA maturation factor (tlr1585) CSB-YP807808TDQ
CSB-EP807808TDQ
CSB-BP807808TDQ
CSB-MP807808TDQ
CSB-EP807808TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermosynechococcus elongatus Photosystem II reaction center protein L (psbL), partial CSB-YP807809TDQ
CSB-EP807809TDQ
CSB-BP807809TDQ
CSB-MP807809TDQ
CSB-EP807809TDQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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