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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant tRNA (Ile)-lysidine synthase (tilS) CSB-YP806149CMN
CSB-EP806149CMN
CSB-BP806149CMN
CSB-MP806149CMN
CSB-EP806149CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 (murA1) CSB-YP806150CMN
CSB-EP806150CMN
CSB-BP806150CMN
CSB-MP806150CMN
CSB-EP806150CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP806151CMN
CSB-EP806151CMN
CSB-BP806151CMN
CSB-MP806151CMN
CSB-EP806151CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane protein insertase YidC (yidC), partial CSB-YP806152CMN1
CSB-EP806152CMN1
CSB-BP806152CMN1
CSB-MP806152CMN1
CSB-EP806152CMN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 2Fe-2S ferredoxin (fdx) CSB-YP806153BMZ
CSB-EP806153BMZ
CSB-BP806153BMZ
CSB-MP806153BMZ
CSB-EP806153BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uncharacterized transporter bbp_532 (bbp_532), partial CSB-YP806154BMZ
CSB-EP806154BMZ
CSB-BP806154BMZ
CSB-MP806154BMZ
CSB-EP806154BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP806155BMZ
CSB-EP806155BMZ
CSB-BP806155BMZ
CSB-MP806155BMZ
CSB-EP806155BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP806156BMZ
CSB-EP806156BMZ
CSB-BP806156BMZ
CSB-MP806156BMZ
CSB-EP806156BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L9 (rplI) CSB-YP806157BMZ
CSB-EP806157BMZ
CSB-BP806157BMZ
CSB-MP806157BMZ
CSB-EP806157BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Putative Holliday junction resolvase (bbp_492) CSB-YP806158BMZ
CSB-EP806158BMZ
CSB-BP806158BMZ
CSB-MP806158BMZ
CSB-EP806158BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP806159BMZ
CSB-EP806159BMZ
CSB-BP806159BMZ
CSB-MP806159BMZ
CSB-EP806159BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Protein TusB (tusB) CSB-YP806160BMZ
CSB-EP806160BMZ
CSB-BP806160BMZ
CSB-MP806160BMZ
CSB-EP806160BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 50S ribosomal protein L16 (rplP) CSB-YP806161BMZ
CSB-EP806161BMZ
CSB-BP806161BMZ
CSB-MP806161BMZ
CSB-EP806161BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae 30S ribosomal protein S5 (rpsE) CSB-YP806162BMZ
CSB-EP806162BMZ
CSB-BP806162BMZ
CSB-MP806162BMZ
CSB-EP806162BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae UPF0250 protein bbp_432 (bbp_432) CSB-YP806163BMZ
CSB-EP806163BMZ
CSB-BP806163BMZ
CSB-MP806163BMZ
CSB-EP806163BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP806164BMZ
CSB-EP806164BMZ
CSB-BP806164BMZ
CSB-MP806164BMZ
CSB-EP806164BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Exodeoxyribonuclease V beta chain (recB), partial CSB-YP806165BMZ
CSB-EP806165BMZ
CSB-BP806165BMZ
CSB-MP806165BMZ
CSB-EP806165BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Sulfurtransferase TusA (tusA) CSB-YP806166BMZ
CSB-EP806166BMZ
CSB-BP806166BMZ
CSB-MP806166BMZ
CSB-EP806166BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP806167BMZ1
CSB-EP806167BMZ1
CSB-BP806167BMZ1
CSB-MP806167BMZ1
CSB-EP806167BMZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Thiol:disulfide interchange protein DsbA (dsbA) CSB-YP806168BMZ
CSB-EP806168BMZ
CSB-BP806168BMZ
CSB-MP806168BMZ
CSB-EP806168BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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