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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum 7-cyano-7-deazaguanine synthase (queC) CSB-YP806229BVW
CSB-EP806229BVW
CSB-BP806229BVW
CSB-MP806229BVW
CSB-EP806229BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Elongation factor P (efp) CSB-YP806230BVW
CSB-EP806230BVW
CSB-BP806230BVW
CSB-MP806230BVW
CSB-EP806230BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable septum site-determining protein MinC (minC) CSB-YP806231BVW
CSB-EP806231BVW
CSB-BP806231BVW
CSB-MP806231BVW
CSB-EP806231BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Guanylate kinase (gmk) CSB-YP806232BVW
CSB-EP806232BVW
CSB-BP806232BVW
CSB-MP806232BVW
CSB-EP806232BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP806233BVW
CSB-EP806233BVW
CSB-BP806233BVW
CSB-MP806233BVW
CSB-EP806233BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable potassium transport system protein kup 2 (kup2), partial CSB-YP806234BVW
CSB-EP806234BVW
CSB-BP806234BVW
CSB-MP806234BVW
CSB-EP806234BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP806235BVW
CSB-EP806235BVW
CSB-BP806235BVW
CSB-MP806235BVW
CSB-EP806235BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Uncharacterized transporter bll3471 (bll3471), partial CSB-YP806236BVW
CSB-EP806236BVW
CSB-BP806236BVW
CSB-MP806236BVW
CSB-EP806236BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Putative thymidine phosphorylase (bll3117), partial CSB-YP806237BVW
CSB-EP806237BVW
CSB-BP806237BVW
CSB-MP806237BVW
CSB-EP806237BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ornithine aminotransferase (rocD) CSB-YP806238BVW
CSB-EP806238BVW
CSB-BP806238BVW
CSB-MP806238BVW
CSB-EP806238BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum ATP-dependent Clp protease adapter protein ClpS 1 (clpS1) CSB-YP806239BVW
CSB-EP806239BVW
CSB-BP806239BVW
CSB-MP806239BVW
CSB-EP806239BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP806240BVW
CSB-EP806240BVW
CSB-BP806240BVW
CSB-MP806240BVW
CSB-EP806240BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Protein SlyX homolog (slyX) CSB-YP806241BVW
CSB-EP806241BVW
CSB-BP806241BVW
CSB-MP806241BVW
CSB-EP806241BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Molybdenum import ATP-binding protein ModC 1 (modC1) CSB-YP806242BVW
CSB-EP806242BVW
CSB-BP806242BVW
CSB-MP806242BVW
CSB-EP806242BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphoglycerate kinase (pgk) CSB-YP806243BVW
CSB-EP806243BVW
CSB-BP806243BVW
CSB-MP806243BVW
CSB-EP806243BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Urease accessory protein UreF (ureF) CSB-YP806244BVW
CSB-EP806244BVW
CSB-BP806244BVW
CSB-MP806244BVW
CSB-EP806244BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Glycerol kinase (glpK) CSB-YP806245BVW
CSB-EP806245BVW
CSB-BP806245BVW
CSB-MP806245BVW
CSB-EP806245BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum DNA gyrase inhibitor YacG (yacG) CSB-YP806246BVW
CSB-EP806246BVW
CSB-BP806246BVW
CSB-MP806246BVW
CSB-EP806246BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum ATP synthase subunit b (atpF), partial CSB-YP806247BVW
CSB-EP806247BVW
CSB-BP806247BVW
CSB-MP806247BVW
CSB-EP806247BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphate import ATP-binding protein PstB (pstB) CSB-YP806248BVW
CSB-EP806248BVW
CSB-BP806248BVW
CSB-MP806248BVW
CSB-EP806248BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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