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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S21 2 (rpsU2) CSB-YP806209BVW
CSB-EP806209BVW
CSB-BP806209BVW
CSB-MP806209BVW
CSB-EP806209BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP806210BVW
CSB-EP806210BVW
CSB-BP806210BVW
CSB-MP806210BVW
CSB-EP806210BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP806211BVW
CSB-EP806211BVW
CSB-BP806211BVW
CSB-MP806211BVW
CSB-EP806211BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Thiazole synthase (thiG) CSB-YP806212BVW
CSB-EP806212BVW
CSB-BP806212BVW
CSB-MP806212BVW
CSB-EP806212BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Ketol-acid reductoisomerase (ilvC) CSB-YP806213BVW
CSB-EP806213BVW
CSB-BP806213BVW
CSB-MP806213BVW
CSB-EP806213BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Histidinol-phosphate aminotransferase 1 (hisC1) CSB-YP806214BVW
CSB-EP806214BVW
CSB-BP806214BVW
CSB-MP806214BVW
CSB-EP806214BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Flagellar P-ring protein 1 (flgI1) CSB-YP806215BVW
CSB-EP806215BVW
CSB-BP806215BVW
CSB-MP806215BVW
CSB-EP806215BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UPF0391 membrane protein bsl5717 (bsl5717), partial CSB-YP806216BVW
CSB-EP806216BVW
CSB-BP806216BVW
CSB-MP806216BVW
CSB-EP806216BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L11 (rplK) CSB-YP806217BVW
CSB-EP806217BVW
CSB-BP806217BVW
CSB-MP806217BVW
CSB-EP806217BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S12 (rpsL) CSB-YP806218BVW
CSB-EP806218BVW
CSB-BP806218BVW
CSB-MP806218BVW
CSB-EP806218BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L22 (rplV) CSB-YP806219BVW
CSB-EP806219BVW
CSB-BP806219BVW
CSB-MP806219BVW
CSB-EP806219BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 30S ribosomal protein S14 (rpsN) CSB-YP806220BVW
CSB-EP806220BVW
CSB-BP806220BVW
CSB-MP806220BVW
CSB-EP806220BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L30 (rpmD) CSB-YP806221BVW
CSB-EP806221BVW
CSB-BP806221BVW
CSB-MP806221BVW
CSB-EP806221BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 50S ribosomal protein L33 (rpmG) CSB-YP806222BVW
CSB-EP806222BVW
CSB-BP806222BVW
CSB-MP806222BVW
CSB-EP806222BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Probable manganese transport protein MntH (mntH), partial CSB-YP806223BVW
CSB-EP806223BVW
CSB-BP806223BVW
CSB-MP806223BVW
CSB-EP806223BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Heme A synthase (ctaA), partial CSB-YP806224BVW
CSB-EP806224BVW
CSB-BP806224BVW
CSB-MP806224BVW
CSB-EP806224BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP806225BVW
CSB-EP806225BVW
CSB-BP806225BVW
CSB-MP806225BVW
CSB-EP806225BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP806226BVW
CSB-EP806226BVW
CSB-BP806226BVW
CSB-MP806226BVW
CSB-EP806226BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Deoxyguanosinetriphosphate triphosphohydrolase-like protein (bll4758) CSB-YP806227BVW
CSB-EP806227BVW
CSB-BP806227BVW
CSB-MP806227BVW
CSB-EP806227BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Phosphopantetheine adenylyltransferase (coaD) CSB-YP806228BVW
CSB-EP806228BVW
CSB-BP806228BVW
CSB-MP806228BVW
CSB-EP806228BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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