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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella oneidensis Integration host factor subunit beta (ihfB) CSB-YP809771SGA
CSB-EP809771SGA
CSB-BP809771SGA
CSB-MP809771SGA
CSB-EP809771SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Serine--tRNA ligase (serS) CSB-YP809772SGA
CSB-EP809772SGA
CSB-BP809772SGA
CSB-MP809772SGA
CSB-EP809772SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Nucleoside diphosphate kinase (ndk) CSB-YP809773SGA
CSB-EP809773SGA
CSB-BP809773SGA
CSB-MP809773SGA
CSB-EP809773SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Acylphosphatase (acyP) CSB-YP809774SGA
CSB-EP809774SGA
CSB-BP809774SGA
CSB-MP809774SGA
CSB-EP809774SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Probable chemoreceptor glutamine deamidase CheD 1 (cheD1) CSB-YP809775SGA
CSB-EP809775SGA
CSB-BP809775SGA
CSB-MP809775SGA
CSB-EP809775SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Integration host factor subunit alpha (ihfA) CSB-YP809776SGA
CSB-EP809776SGA
CSB-BP809776SGA
CSB-MP809776SGA
CSB-EP809776SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP809777SGA
CSB-EP809777SGA
CSB-BP809777SGA
CSB-MP809777SGA
CSB-EP809777SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UPF0267 protein SO_1922 (SO_1922) CSB-YP809778SGA
CSB-EP809778SGA
CSB-BP809778SGA
CSB-MP809778SGA
CSB-EP809778SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP809779SGA
CSB-EP809779SGA
CSB-BP809779SGA
CSB-MP809779SGA
CSB-EP809779SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glutamine--tRNA ligase (glnS), partial CSB-YP809780SGA
CSB-EP809780SGA
CSB-BP809780SGA
CSB-MP809780SGA
CSB-EP809780SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis tRNA (Ile)-lysidine synthase (tilS) CSB-YP809781SGA
CSB-EP809781SGA
CSB-BP809781SGA
CSB-MP809781SGA
CSB-EP809781SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Ribosome-recycling factor (frr) CSB-YP809782SGA
CSB-EP809782SGA
CSB-BP809782SGA
CSB-MP809782SGA
CSB-EP809782SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis [Protein-PII] uridylyltransferase (glnD), partial CSB-YP809783SGA
CSB-EP809783SGA
CSB-BP809783SGA
CSB-MP809783SGA
CSB-EP809783SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP809784SGA
CSB-EP809784SGA
CSB-BP809784SGA
CSB-MP809784SGA
CSB-EP809784SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UPF0125 protein SO_1475 (SO_1475) CSB-YP809785SGA
CSB-EP809785SGA
CSB-BP809785SGA
CSB-MP809785SGA
CSB-EP809785SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Elongation factor 4 (lepA), partial CSB-YP809786SGA
CSB-EP809786SGA
CSB-BP809786SGA
CSB-MP809786SGA
CSB-EP809786SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis DNA mismatch repair protein mutH (mutH) CSB-YP809787SGA
CSB-EP809787SGA
CSB-BP809787SGA
CSB-MP809787SGA
CSB-EP809787SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Undecaprenyl-diphosphatase 1 (uppP1), partial CSB-YP809788SGA
CSB-EP809788SGA
CSB-BP809788SGA
CSB-MP809788SGA
CSB-EP809788SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Purine nucleoside phosphorylase DeoD-type 2 (deoD2) CSB-YP809789SGA
CSB-EP809789SGA
CSB-BP809789SGA
CSB-MP809789SGA
CSB-EP809789SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP809790SGA
CSB-EP809790SGA
CSB-BP809790SGA
CSB-MP809790SGA
CSB-EP809790SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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