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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oceanobacillus iheyensis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP809831OAB
CSB-EP809831OAB
CSB-BP809831OAB
CSB-MP809831OAB
CSB-EP809831OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Aspartate--tRNA ligase (aspS), partial CSB-YP809832OAB
CSB-EP809832OAB
CSB-BP809832OAB
CSB-MP809832OAB
CSB-EP809832OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 30S ribosomal protein S20 (rpsT) CSB-YP809833OAB
CSB-EP809833OAB
CSB-BP809833OAB
CSB-MP809833OAB
CSB-EP809833OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Deoxyribose-phosphate aldolase 1 (deoC1) CSB-YP809834OAB
CSB-EP809834OAB
CSB-BP809834OAB
CSB-MP809834OAB
CSB-EP809834OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis N utilization substance protein B homolog (nusB) CSB-YP809835OAB
CSB-EP809835OAB
CSB-BP809835OAB
CSB-MP809835OAB
CSB-EP809835OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis DNA polymerase IV (dinB) CSB-YP809836OAB
CSB-EP809836OAB
CSB-BP809836OAB
CSB-MP809836OAB
CSB-EP809836OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Transcription attenuation protein mtrB (mtrB) CSB-YP809837OAB
CSB-EP809837OAB
CSB-BP809837OAB
CSB-MP809837OAB
CSB-EP809837OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Asparagine--tRNA ligase (asnS) CSB-YP809838OAB
CSB-EP809838OAB
CSB-BP809838OAB
CSB-MP809838OAB
CSB-EP809838OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis UPF0154 protein OB1676 (OB1676), partial CSB-YP809839OAB
CSB-EP809839OAB
CSB-BP809839OAB
CSB-MP809839OAB
CSB-EP809839OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Sensor protein lytS (lytS), partial CSB-YP809840OAB1
CSB-EP809840OAB1
CSB-BP809840OAB1
CSB-MP809840OAB1
CSB-EP809840OAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Ribosome-binding factor A (rbfA) CSB-YP809841OAB
CSB-EP809841OAB
CSB-BP809841OAB
CSB-MP809841OAB
CSB-EP809841OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Ribosome-recycling factor (frr) CSB-YP809842OAB
CSB-EP809842OAB
CSB-BP809842OAB
CSB-MP809842OAB
CSB-EP809842OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 30S ribosomal protein S16 (rpsP) CSB-YP809843OAB
CSB-EP809843OAB
CSB-BP809843OAB
CSB-MP809843OAB
CSB-EP809843OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Putative ribosome biogenesis GTPase RsgA 2 (rsgA2) CSB-YP809844OAB
CSB-EP809844OAB
CSB-BP809844OAB
CSB-MP809844OAB
CSB-EP809844OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Lipoprotein signal peptidase (lspA), partial CSB-YP809845OAB1
CSB-EP809845OAB1
CSB-BP809845OAB1
CSB-MP809845OAB1
CSB-EP809845OAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Protein MraZ (mraZ) CSB-YP809846OAB
CSB-EP809846OAB
CSB-BP809846OAB
CSB-MP809846OAB
CSB-EP809846OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis UPF0356 protein OB1409 (OB1409) CSB-YP809847OAB
CSB-EP809847OAB
CSB-BP809847OAB
CSB-MP809847OAB
CSB-EP809847OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Lipoyl synthase (lipA) CSB-YP809848OAB
CSB-EP809848OAB
CSB-BP809848OAB
CSB-MP809848OAB
CSB-EP809848OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP809849OAB
CSB-EP809849OAB
CSB-BP809849OAB
CSB-MP809849OAB
CSB-EP809849OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP809850OAB
CSB-EP809850OAB
CSB-BP809850OAB
CSB-MP809850OAB
CSB-EP809850OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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