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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella oneidensis Maf-like protein SO_4095 (SO_4095) CSB-YP809751SGA
CSB-EP809751SGA
CSB-BP809751SGA
CSB-MP809751SGA
CSB-EP809751SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP809752SGA
CSB-EP809752SGA
CSB-BP809752SGA
CSB-MP809752SGA
CSB-EP809752SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Adenylosuccinate synthetase (purA) CSB-YP809753SGA
CSB-EP809753SGA
CSB-BP809753SGA
CSB-MP809753SGA
CSB-EP809753SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis PKHD-type hydroxylase SO_3913 (SO_3913) CSB-YP809754SGA
CSB-EP809754SGA
CSB-BP809754SGA
CSB-MP809754SGA
CSB-EP809754SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Molybdenum import ATP-binding protein ModC (modC) CSB-YP809755SGA
CSB-EP809755SGA
CSB-BP809755SGA
CSB-MP809755SGA
CSB-EP809755SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP809756SGA
CSB-EP809756SGA
CSB-BP809756SGA
CSB-MP809756SGA
CSB-EP809756SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP809757SGA
CSB-EP809757SGA
CSB-BP809757SGA
CSB-MP809757SGA
CSB-EP809757SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Hemin import ATP-binding protein HmuV (hmuV) CSB-YP809758SGA
CSB-EP809758SGA
CSB-BP809758SGA
CSB-MP809758SGA
CSB-EP809758SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis DnaJ-like protein DjlA (djlA), partial CSB-YP809759SGA
CSB-EP809759SGA
CSB-BP809759SGA
CSB-MP809759SGA
CSB-EP809759SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Serine hydroxymethyltransferase (glyA) CSB-YP809760SGA
CSB-EP809760SGA
CSB-BP809760SGA
CSB-MP809760SGA
CSB-EP809760SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Probable transcriptional regulatory protein SO_3401 (SO_3401) CSB-YP809761SGA
CSB-EP809761SGA
CSB-BP809761SGA
CSB-MP809761SGA
CSB-EP809761SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP809762SGA
CSB-EP809762SGA
CSB-BP809762SGA
CSB-MP809762SGA
CSB-EP809762SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Serine/threonine transporter sstT (sstT), partial CSB-YP809763SGA
CSB-EP809763SGA
CSB-BP809763SGA
CSB-MP809763SGA
CSB-EP809763SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Anthranilate phosphoribosyltransferase (trpD) CSB-YP809764SGA
CSB-EP809764SGA
CSB-BP809764SGA
CSB-MP809764SGA
CSB-EP809764SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Fatty acid metabolism regulator protein (fadR) CSB-YP809765SGA
CSB-EP809765SGA
CSB-BP809765SGA
CSB-MP809765SGA
CSB-EP809765SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Biotin synthase (bioB) CSB-YP809766SGA
CSB-EP809766SGA
CSB-BP809766SGA
CSB-MP809766SGA
CSB-EP809766SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis N-succinylarginine dihydrolase (astB) CSB-YP809767SGA
CSB-EP809767SGA
CSB-BP809767SGA
CSB-MP809767SGA
CSB-EP809767SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Putative arginyl-tRNA--protein transferase (ate) CSB-YP809768SGA
CSB-EP809768SGA
CSB-BP809768SGA
CSB-MP809768SGA
CSB-EP809768SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Cell division protein ZapC (zapC) CSB-YP809769SGA
CSB-EP809769SGA
CSB-BP809769SGA
CSB-MP809769SGA
CSB-EP809769SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Phosphomethylpyrimidine synthase (thiC), partial CSB-YP809770SGA
CSB-EP809770SGA
CSB-BP809770SGA
CSB-MP809770SGA
CSB-EP809770SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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