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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella oneidensis Ribose-5-phosphate isomerase A (rpiA) CSB-YP809791SGA
CSB-EP809791SGA
CSB-BP809791SGA
CSB-MP809791SGA
CSB-EP809791SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Na (+)-translocating NADH-quinone reductase subunit D (nqrD), partial CSB-YP809792SGA1
CSB-EP809792SGA1
CSB-BP809792SGA1
CSB-MP809792SGA1
CSB-EP809792SGA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP809793SGA
CSB-EP809793SGA
CSB-BP809793SGA
CSB-MP809793SGA
CSB-EP809793SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC (rlmC) CSB-YP809794SGA
CSB-EP809794SGA
CSB-BP809794SGA
CSB-MP809794SGA
CSB-EP809794SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP809795SGA
CSB-EP809795SGA
CSB-BP809795SGA
CSB-MP809795SGA
CSB-EP809795SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP809796SGA
CSB-EP809796SGA
CSB-BP809796SGA
CSB-MP809796SGA
CSB-EP809796SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP809797SGA
CSB-EP809797SGA
CSB-BP809797SGA
CSB-MP809797SGA
CSB-EP809797SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP809798SGA
CSB-EP809798SGA
CSB-BP809798SGA
CSB-MP809798SGA
CSB-EP809798SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis ATP-dependent RNA helicase rhlB (rhlB) CSB-YP809799SGA
CSB-EP809799SGA
CSB-BP809799SGA
CSB-MP809799SGA
CSB-EP809799SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis UPF0313 protein SO_0311 (SO_0311), partial CSB-YP809800SGA
CSB-EP809800SGA
CSB-BP809800SGA
CSB-MP809800SGA
CSB-EP809800SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Shikimate kinase (aroK) CSB-YP809801SGA
CSB-EP809801SGA
CSB-BP809801SGA
CSB-MP809801SGA
CSB-EP809801SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP809802SGA
CSB-EP809802SGA
CSB-BP809802SGA
CSB-MP809802SGA
CSB-EP809802SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 30S ribosomal protein S8 (rpsH) CSB-YP809803SGA
CSB-EP809803SGA
CSB-BP809803SGA
CSB-MP809803SGA
CSB-EP809803SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 50S ribosomal protein L16 (rplP) CSB-YP809804SGA
CSB-EP809804SGA
CSB-BP809804SGA
CSB-MP809804SGA
CSB-EP809804SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis 50S ribosomal protein L3 (rplC) CSB-YP809805SGA
CSB-EP809805SGA
CSB-BP809805SGA
CSB-MP809805SGA
CSB-EP809805SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP809806SGA
CSB-EP809806SGA
CSB-BP809806SGA
CSB-MP809806SGA
CSB-EP809806SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Elongation factor Tu 1 (tuf1) CSB-YP809807SGA
CSB-EP809807SGA
CSB-BP809807SGA
CSB-MP809807SGA
CSB-EP809807SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Probable chorismate--pyruvate lyase (ubiC) CSB-YP809808SGA
CSB-EP809808SGA
CSB-BP809808SGA
CSB-MP809808SGA
CSB-EP809808SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Imidazolonepropionase (hutI) CSB-YP809809SGA
CSB-EP809809SGA
CSB-BP809809SGA
CSB-MP809809SGA
CSB-EP809809SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella oneidensis Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP809810SGA
CSB-EP809810SGA
CSB-BP809810SGA
CSB-MP809810SGA
CSB-EP809810SGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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