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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP810031CQG
CSB-EP810031CQG
CSB-BP810031CQG
CSB-MP810031CQG
CSB-EP810031CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein FolD (folD) CSB-YP810032CQG
CSB-EP810032CQG
CSB-BP810032CQG
CSB-MP810032CQG
CSB-EP810032CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized transporter CE0595 (CE0595), partial CSB-YP810033CQG
CSB-EP810033CQG
CSB-BP810033CQG
CSB-MP810033CQG
CSB-EP810033CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L13 (rplM) CSB-YP810034CQG
CSB-EP810034CQG
CSB-BP810034CQG
CSB-MP810034CQG
CSB-EP810034CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-1 (infA) CSB-YP810035CQG
CSB-EP810035CQG
CSB-BP810035CQG
CSB-MP810035CQG
CSB-EP810035CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L30 (rpmD) CSB-YP810036CQG
CSB-EP810036CQG
CSB-BP810036CQG
CSB-MP810036CQG
CSB-EP810036CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP810037CQG
CSB-EP810037CQG
CSB-BP810037CQG
CSB-MP810037CQG
CSB-EP810037CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L34 (rpmH) CSB-YP810038CQG
CSB-EP810038CQG
CSB-BP810038CQG
CSB-MP810038CQG
CSB-EP810038CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carboxylate-amine ligase CE2886 (CE2886) CSB-YP810039CQG
CSB-EP810039CQG
CSB-BP810039CQG
CSB-MP810039CQG
CSB-EP810039CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein pyrR (pyrR) CSB-YP810040CQG
CSB-EP810040CQG
CSB-BP810040CQG
CSB-MP810040CQG
CSB-EP810040CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase 1 (def1) CSB-YP810041CQG
CSB-EP810041CQG
CSB-BP810041CQG
CSB-MP810041CQG
CSB-EP810041CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP810042CQG
CSB-EP810042CQG
CSB-BP810042CQG
CSB-MP810042CQG
CSB-EP810042CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sec-independent protein translocase protein TatA (tatA), partial CSB-YP810043CQG
CSB-EP810043CQG
CSB-BP810043CQG
CSB-MP810043CQG
CSB-EP810043CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylglutamate kinase (argB) CSB-YP810044CQG
CSB-EP810044CQG
CSB-BP810044CQG
CSB-MP810044CQG
CSB-EP810044CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP810045CQG
CSB-EP810045CQG
CSB-BP810045CQG
CSB-MP810045CQG
CSB-EP810045CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP810046CQG
CSB-EP810046CQG
CSB-BP810046CQG
CSB-MP810046CQG
CSB-EP810046CQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Leu/Ile/Val-binding protein homolog 5 (BRA1193, BS1330_II1184) CSB-YP810047BMQ
CSB-EP810047BMQ
CSB-BP810047BMQ
CSB-MP810047BMQ
CSB-EP810047BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 High-affinity zinc uptake system protein znuA (znuA) CSB-YP810048BMQ
CSB-EP810048BMQ
CSB-BP810048BMQ
CSB-MP810048BMQ
CSB-EP810048BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Dihydrodipicolinate reductase (dapB) CSB-YP810049BMQ
CSB-EP810049BMQ
CSB-BP810049BMQ
CSB-MP810049BMQ
CSB-EP810049BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 D-aminopeptidase (dap), partial CSB-YP810050BMQ
CSB-EP810050BMQ
CSB-BP810050BMQ
CSB-MP810050BMQ
CSB-EP810050BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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