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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis biovar 1 50S ribosomal protein L11 (rplK) CSB-YP810071BMQ
CSB-EP810071BMQ
CSB-BP810071BMQ
CSB-MP810071BMQ
CSB-EP810071BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Elongation factor G (fusA), partial CSB-YP810072BMQ
CSB-EP810072BMQ
CSB-BP810072BMQ
CSB-MP810072BMQ
CSB-EP810072BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 50S ribosomal protein L16 (rplP) CSB-YP810073BMQ
CSB-EP810073BMQ
CSB-BP810073BMQ
CSB-MP810073BMQ
CSB-EP810073BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP810074BMQ
CSB-EP810074BMQ
CSB-BP810074BMQ
CSB-MP810074BMQ
CSB-EP810074BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Undecaprenyl pyrophosphate synthase (uppS) CSB-YP810075BMQ
CSB-EP810075BMQ
CSB-BP810075BMQ
CSB-MP810075BMQ
CSB-EP810075BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP810076BMQ
CSB-EP810076BMQ
CSB-BP810076BMQ
CSB-MP810076BMQ
CSB-EP810076BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Queuine tRNA-ribosyltransferase (tgt) CSB-YP810077BMQ
CSB-EP810077BMQ
CSB-BP810077BMQ
CSB-MP810077BMQ
CSB-EP810077BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UPF0283 membrane protein BR1033/BS1330_I1029 (BR1033, BS1330_I1029), partial CSB-YP810078BMQ1
CSB-EP810078BMQ1
CSB-BP810078BMQ1
CSB-MP810078BMQ1
CSB-EP810078BMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP810079BMQ
CSB-EP810079BMQ
CSB-BP810079BMQ
CSB-MP810079BMQ
CSB-EP810079BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP810080BMQ
CSB-EP810080BMQ
CSB-BP810080BMQ
CSB-MP810080BMQ
CSB-EP810080BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP810081BMQ1
CSB-EP810081BMQ1
CSB-BP810081BMQ1
CSB-MP810081BMQ1
CSB-EP810081BMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Heme A synthase (ctaA), partial CSB-YP810082BMQ
CSB-EP810082BMQ
CSB-BP810082BMQ
CSB-MP810082BMQ
CSB-EP810082BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Dihydrodipicolinate synthase (dapA) CSB-YP810083BMQ
CSB-EP810083BMQ
CSB-BP810083BMQ
CSB-MP810083BMQ
CSB-EP810083BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Cytochrome c oxidase assembly protein CtaG (ctaG), partial CSB-YP810084BMQ
CSB-EP810084BMQ
CSB-BP810084BMQ
CSB-MP810084BMQ
CSB-EP810084BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP810085BMQ
CSB-EP810085BMQ
CSB-BP810085BMQ
CSB-MP810085BMQ
CSB-EP810085BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Uncharacterized protein BR0337/BS1330_I0338 (BR0337, BS1330_I0338) CSB-YP810086BMQ
CSB-EP810086BMQ
CSB-BP810086BMQ
CSB-MP810086BMQ
CSB-EP810086BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP810087BMQ
CSB-EP810087BMQ
CSB-BP810087BMQ
CSB-MP810087BMQ
CSB-EP810087BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Urease accessory protein UreE 1 (ureE1) CSB-YP810088BMQ
CSB-EP810088BMQ
CSB-BP810088BMQ
CSB-MP810088BMQ
CSB-EP810088BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Maf-like protein BR0248/BS1330_I0249 (maf-1) CSB-YP810089BMQ
CSB-EP810089BMQ
CSB-BP810089BMQ
CSB-MP810089BMQ
CSB-EP810089BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Nicotinate phosphoribosyltransferase (pncB) CSB-YP810090BMQ
CSB-EP810090BMQ
CSB-BP810090BMQ
CSB-MP810090BMQ
CSB-EP810090BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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