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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella suis biovar 1 Uncharacterized protein BRA0921/BS1330_II0913 (BRA0921, BS1330_II0913) CSB-YP810051BMQ
CSB-EP810051BMQ
CSB-BP810051BMQ
CSB-MP810051BMQ
CSB-EP810051BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Protein CrcB homolog 3 (crcB3), partial CSB-YP810052BMQ1
CSB-EP810052BMQ1
CSB-BP810052BMQ1
CSB-MP810052BMQ1
CSB-EP810052BMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Fe (3+) ions import ATP-binding protein FbpC (fbpC) CSB-YP810053BMQ
CSB-EP810053BMQ
CSB-BP810053BMQ
CSB-MP810053BMQ
CSB-EP810053BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 DNA polymerase IV (dinB) CSB-YP810054BMQ
CSB-EP810054BMQ
CSB-BP810054BMQ
CSB-MP810054BMQ
CSB-EP810054BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Homoserine O-succinyltransferase (metA) CSB-YP810055BMQ
CSB-EP810055BMQ
CSB-BP810055BMQ
CSB-MP810055BMQ
CSB-EP810055BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UPF0423 protein BRA0381/BS1330_II0378 (BRA0381, BS1330_II0378) CSB-YP810056BMQ
CSB-EP810056BMQ
CSB-BP810056BMQ
CSB-MP810056BMQ
CSB-EP810056BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Protein norD (norD), partial CSB-YP810057BMQ
CSB-EP810057BMQ
CSB-BP810057BMQ
CSB-MP810057BMQ
CSB-EP810057BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Flagellar L-ring protein (flgH) CSB-YP810058BMQ
CSB-EP810058BMQ
CSB-BP810058BMQ
CSB-MP810058BMQ
CSB-EP810058BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP810059BMQ
CSB-EP810059BMQ
CSB-BP810059BMQ
CSB-MP810059BMQ
CSB-EP810059BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Tryptophan synthase alpha chain (trpA) CSB-YP810060BMQ
CSB-EP810060BMQ
CSB-BP810060BMQ
CSB-MP810060BMQ
CSB-EP810060BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Protein-export protein SecB (secB) CSB-YP810061BMQ
CSB-EP810061BMQ
CSB-BP810061BMQ
CSB-MP810061BMQ
CSB-EP810061BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Aquaporin Z (aqpZ), partial CSB-YP810062BMQ1
CSB-EP810062BMQ1
CSB-BP810062BMQ1
CSB-MP810062BMQ1
CSB-EP810062BMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Malate dehydrogenase (mdh) CSB-YP810063BMQ
CSB-EP810063BMQ
CSB-BP810063BMQ
CSB-MP810063BMQ
CSB-EP810063BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 DNA translocase FtsK (ftsK), partial CSB-YP810064BMQ
CSB-EP810064BMQ
CSB-BP810064BMQ
CSB-MP810064BMQ
CSB-EP810064BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Leucine--tRNA ligase (leuS), partial CSB-YP810065BMQ
CSB-EP810065BMQ
CSB-BP810065BMQ
CSB-MP810065BMQ
CSB-EP810065BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP810066BMQ1
CSB-EP810066BMQ1
CSB-BP810066BMQ1
CSB-MP810066BMQ1
CSB-EP810066BMQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Cell cycle response regulator CtrA (ctrA) CSB-YP810067BMQ
CSB-EP810067BMQ
CSB-BP810067BMQ
CSB-MP810067BMQ
CSB-EP810067BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP810068BMQ
CSB-EP810068BMQ
CSB-BP810068BMQ
CSB-MP810068BMQ
CSB-EP810068BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 UPF0303 protein BR1411/BS1330_I1405 (BR1411, BS1330_I1405) CSB-YP810069BMQ
CSB-EP810069BMQ
CSB-BP810069BMQ
CSB-MP810069BMQ
CSB-EP810069BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Urease accessory protein UreG 2 (ureG2) CSB-YP810070BMQ
CSB-EP810070BMQ
CSB-BP810070BMQ
CSB-MP810070BMQ
CSB-EP810070BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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