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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O6:H1 Alpha-hemolysin translocation ATP-binding protein HlyB (hlyB), partial CSB-YP809951EGX1
CSB-EP809951EGX1
CSB-BP809951EGX1
CSB-MP809951EGX1
CSB-EP809951EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agmatinase (speB) CSB-YP809952EGX
CSB-EP809952EGX
CSB-BP809952EGX
CSB-MP809952EGX
CSB-EP809952EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ) CSB-YP809953EGX
CSB-EP809953EGX
CSB-BP809953EGX
CSB-MP809953EGX
CSB-EP809953EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP809954EGX
CSB-EP809954EGX
CSB-BP809954EGX
CSB-MP809954EGX
CSB-EP809954EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0125 protein YfjF (yfjF) CSB-YP809955EGX
CSB-EP809955EGX
CSB-BP809955EGX
CSB-MP809955EGX
CSB-EP809955EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA1 (Val) (adenine (37)-N6)-methyltransferase (yfiC) CSB-YP809956EGX
CSB-EP809956EGX
CSB-BP809956EGX
CSB-MP809956EGX
CSB-EP809956EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP809957EGX
CSB-EP809957EGX
CSB-BP809957EGX
CSB-MP809957EGX
CSB-EP809957EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP809958EGX
CSB-EP809958EGX
CSB-BP809958EGX
CSB-MP809958EGX
CSB-EP809958EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multiphosphoryl transfer protein (fryA), partial CSB-YP809959EGX
CSB-EP809959EGX
CSB-BP809959EGX
CSB-MP809959EGX
CSB-EP809959EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP809960EGX
CSB-EP809960EGX
CSB-BP809960EGX
CSB-MP809960EGX
CSB-EP809960EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP809961EGX
CSB-EP809961EGX
CSB-BP809961EGX
CSB-MP809961EGX
CSB-EP809961EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP809962EGX1
CSB-EP809962EGX1
CSB-BP809962EGX1
CSB-MP809962EGX1
CSB-EP809962EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-rhamnonate dehydratase (rhmD) CSB-YP809963EGX
CSB-EP809963EGX
CSB-BP809963EGX
CSB-MP809963EGX
CSB-EP809963EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0324 inner membrane protein yeiH (yeiH), partial CSB-YP809964EGX
CSB-EP809964EGX
CSB-BP809964EGX
CSB-MP809964EGX
CSB-EP809964EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galactose/methyl galactoside import ATP-binding protein MglA (mglA) CSB-YP809965EGX
CSB-EP809965EGX
CSB-BP809965EGX
CSB-MP809965EGX
CSB-EP809965EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP809966EGX
CSB-EP809966EGX
CSB-BP809966EGX
CSB-MP809966EGX
CSB-EP809966EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP809967EGX
CSB-EP809967EGX
CSB-BP809967EGX
CSB-MP809967EGX
CSB-EP809967EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cobalamin synthase (cobS), partial CSB-YP809968EGX1
CSB-EP809968EGX1
CSB-BP809968EGX1
CSB-MP809968EGX1
CSB-EP809968EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfoxide reductase heme-binding subunit YedZ (yedZ), partial CSB-YP809969EGX1
CSB-EP809969EGX1
CSB-BP809969EGX1
CSB-MP809969EGX1
CSB-EP809969EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Universal stress protein C (uspC) CSB-YP809970EGX
CSB-EP809970EGX
CSB-BP809970EGX
CSB-MP809970EGX
CSB-EP809970EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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