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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. japonica Heat stress transcription factor A-2d (HSFA2D) CSB-YP810191OFG
CSB-EP810191OFG
CSB-BP810191OFG
CSB-MP810191OFG
CSB-EP810191OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum NAD (P)H:quinone oxidoreductase CSB-YP810192FIG
CSB-EP810192FIG
CSB-BP810192FIG
CSB-MP810192FIG
CSB-EP810192FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NADH-ubiquinone oxidoreductase chain 6 (nd6), partial CSB-YP810193CXX1
CSB-EP810193CXX1
CSB-BP810193CXX1
CSB-MP810193CXX1
CSB-EP810193CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aerodramus vulcanorum Cytochrome b (MT-CYB), partial CSB-YP810194AHAI
CSB-EP810194AHAI
CSB-BP810194AHAI
CSB-MP810194AHAI
CSB-EP810194AHAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synbranchus marmoratus Cytochrome b (mt-cyb), partial CSB-YP810195SIAD
CSB-EP810195SIAD
CSB-BP810195SIAD
CSB-MP810195SIAD
CSB-EP810195SIAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vespertilio superans Cytochrome b (MT-CYB), partial CSB-YP810196VBAC
CSB-EP810196VBAC
CSB-BP810196VBAC
CSB-MP810196VBAC
CSB-EP810196VBAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Miniopterus fuliginosus Cytochrome b (MT-CYB), partial CSB-YP810197MHAN
CSB-EP810197MHAN
CSB-BP810197MHAN
CSB-MP810197MHAN
CSB-EP810197MHAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus roxburghii Maturase K (matK), partial CSB-YP810198PJAB
CSB-EP810198PJAB
CSB-BP810198PJAB
CSB-MP810198PJAB
CSB-EP810198PJAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus pungens Maturase K (matK), partial CSB-YP810199PJAC
CSB-EP810199PJAC
CSB-BP810199PJAC
CSB-MP810199PJAC
CSB-EP810199PJAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ephedra sinica Cytochrome b559 subunit beta (psbF), partial CSB-YP810200EBAI
CSB-EP810200EBAI
CSB-BP810200EBAI
CSB-MP810200EBAI
CSB-EP810200EBAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii 50S ribosomal protein L23, chloroplastic (rpl23) CSB-YP810201DSJ
CSB-EP810201DSJ
CSB-BP810201DSJ
CSB-MP810201DSJ
CSB-EP810201DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Putative 30S ribosomal S2-like protein (rps2-2) CSB-YP810202DSJ
CSB-EP810202DSJ
CSB-BP810202DSJ
CSB-MP810202DSJ
CSB-EP810202DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arnica dealbata NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810203AHAJ
CSB-EP810203AHAJ
CSB-BP810203AHAJ
CSB-MP810203AHAJ
CSB-EP810203AHAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810204VBAD
CSB-EP810204VBAD
CSB-BP810204VBAD
CSB-MP810204VBAD
CSB-EP810204VBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tagetes erecta NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810205TDAP
CSB-EP810205TDAP
CSB-BP810205TDAP
CSB-MP810205TDAP
CSB-EP810205TDAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Smallanthus microcephalus NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810206SIAE
CSB-EP810206SIAE
CSB-BP810206SIAE
CSB-MP810206SIAE
CSB-EP810206SIAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810207RDAD
CSB-EP810207RDAD
CSB-BP810207RDAD
CSB-MP810207RDAD
CSB-EP810207RDAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parthenium hysterophorus NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810208PJAD
CSB-EP810208PJAD
CSB-BP810208PJAD
CSB-MP810208PJAD
CSB-EP810208PJAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Melanthera integrifolia NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810209MHAO
CSB-EP810209MHAO
CSB-BP810209MHAO
CSB-MP810209MHAO
CSB-EP810209MHAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP810210ICAM
CSB-EP810210ICAM
CSB-BP810210ICAM
CSB-MP810210ICAM
CSB-EP810210ICAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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