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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Thioredoxin-T (TrxT) CSB-YP810251DLU
CSB-EP810251DLU
CSB-BP810251DLU
CSB-MP810251DLU
CSB-EP810251DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Probable cathepsin C (PF11_0174), partial CSB-YP810252EWP
CSB-EP810252EWP
CSB-BP810252EWP
CSB-MP810252EWP
CSB-EP810252EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum STI1-like protein (PF14_0324), partial CSB-YP810253EWP
CSB-EP810253EWP
CSB-BP810253EWP
CSB-MP810253EWP
CSB-EP810253EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Protein PF14_0175 (PF14_0175), partial CSB-YP810254EWP
CSB-EP810254EWP
CSB-BP810254EWP
CSB-MP810254EWP
CSB-EP810254EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Uncharacterized protein PF14_0045 (PF14_0045), partial CSB-YP810255EWP
CSB-EP810255EWP
CSB-BP810255EWP
CSB-MP810255EWP
CSB-EP810255EWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein Turandot Z (TotZ) CSB-YP810256DLU
CSB-EP810256DLU
CSB-BP810256DLU
CSB-MP810256DLU
CSB-EP810256DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Soluble guanylate cyclase 88E (Gyc88E), partial CSB-YP810257DLU
CSB-EP810257DLU
CSB-BP810257DLU
CSB-MP810257DLU
CSB-EP810257DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Peptidoglycan-recognition protein LB (PGRP-LB) CSB-YP810258DLU
CSB-EP810258DLU
CSB-BP810258DLU
CSB-MP810258DLU
CSB-EP810258DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Slowpoke-binding protein (Slob), partial CSB-YP810259DLU
CSB-EP810259DLU
CSB-BP810259DLU
CSB-MP810259DLU
CSB-EP810259DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Claspin homolog (CG32251), partial CSB-YP810260DLU
CSB-EP810260DLU
CSB-BP810260DLU
CSB-MP810260DLU
CSB-EP810260DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Vanin-like protein 2 (CG32751) CSB-YP810261DLU
CSB-EP810261DLU
CSB-BP810261DLU
CSB-MP810261DLU
CSB-EP810261DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ras guanine nucleotide exchange factor I (gefI), partial CSB-YP810262DKK
CSB-EP810262DKK
CSB-BP810262DKK
CSB-MP810262DKK
CSB-EP810262DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus vexillum Conotoxin VxVIB CSB-YP810263DWK
CSB-EP810263DWK
CSB-BP810263DWK
CSB-MP810263DWK
CSB-EP810263DWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein CASC2, isoforms 1/2 (CASC2) CSB-YP810264HU
CSB-EP810264HU
CSB-BP810264HU
CSB-MP810264HU
CSB-EP810264HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Keratin-associated protein 11-1 (KRTAP11-1) CSB-YP810266HU
CSB-EP810266HU
CSB-BP810266HU
CSB-MP810266HU
CSB-EP810266HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human MYC-induced nuclear antigen (MINA) CSB-YP810267HU
CSB-EP810267HU
CSB-BP810267HU
CSB-MP810267HU
CSB-EP810267HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Spermatogenesis-associated protein 2-like protein (SPATA2L) CSB-YP810268HU
CSB-EP810268HU
CSB-BP810268HU
CSB-MP810268HU
CSB-EP810268HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Carabin (TBC1D10C) CSB-YP810269HU
CSB-EP810269HU
CSB-BP810269HU
CSB-MP810269HU
CSB-EP810269HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Coiled-coil domain-containing protein 71 (CCDC71) CSB-YP810270HU
CSB-EP810270HU
CSB-BP810270HU
CSB-MP810270HU
CSB-EP810270HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human WD repeat-containing protein 49 (WDR49) CSB-YP810271HU
CSB-EP810271HU
CSB-BP810271HU
CSB-MP810271HU
CSB-EP810271HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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