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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Beta-galactosidase BgaA (bgaA), partial CSB-YP810131TNQ
CSB-EP810131TNQ
CSB-BP810131TNQ
CSB-MP810131TNQ
CSB-EP810131TNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. O-acyltransferase WSD (wax-dgaT) CSB-YP810132AWW
CSB-EP810132AWW
CSB-BP810132AWW
CSB-MP810132AWW
CSB-EP810132AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Probable manganese transport protein MntH (mntH), partial CSB-YP810133LMS
CSB-EP810133LMS
CSB-BP810133LMS
CSB-MP810133LMS
CSB-EP810133LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP810134SYN
CSB-EP810134SYN
CSB-BP810134SYN
CSB-MP810134SYN
CSB-EP810134SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endo-1,4-beta-xylanase A (xynA), partial CSB-YP810135CML
CSB-EP810135CML
CSB-BP810135CML
CSB-MP810135CML
CSB-EP810135CML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP810136FPY
CSB-EP810136FPY
CSB-BP810136FPY
CSB-MP810136FPY
CSB-EP810136FPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP810137DNW
CSB-EP810137DNW
CSB-BP810137DNW
CSB-MP810137DNW
CSB-EP810137DNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hominis 50S ribosomal protein L2 (rplB) CSB-YP810138MWJ
CSB-EP810138MWJ
CSB-BP810138MWJ
CSB-MP810138MWJ
CSB-EP810138MWJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Holliday junction resolvase recU (recU) CSB-YP810139FKZ
CSB-EP810139FKZ
CSB-BP810139FKZ
CSB-MP810139FKZ
CSB-EP810139FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylacetone-cleaving enzyme (dke1) CSB-YP810140AWT
CSB-EP810140AWT
CSB-BP810140AWT
CSB-MP810140AWT
CSB-EP810140AWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodovulum sulfidophilum Dimethylsulfide dehydrogenase subunit alpha (ddhA), partial CSB-YP810141RMF
CSB-EP810141RMF
CSB-BP810141RMF
CSB-MP810141RMF
CSB-EP810141RMF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Inorganic phosphate transporter 1-2 (PTH1-2), partial CSB-YP810142OFG
CSB-EP810142OFG
CSB-BP810142OFG
CSB-MP810142OFG
CSB-EP810142OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Zygote-specific protein 3 (ZYS3) CSB-YP810143DSJ
CSB-EP810143DSJ
CSB-BP810143DSJ
CSB-MP810143DSJ
CSB-EP810143DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NEP1-interacting protein 1 (NIP1), partial CSB-YP810144DOA1
CSB-EP810144DOA1
CSB-BP810144DOA1
CSB-MP810144DOA1
CSB-EP810144DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carica papaya S-adenosylmethionine synthase (SAMS) CSB-YP810145DQH
CSB-EP810145DQH
CSB-BP810145DQH
CSB-MP810145DQH
CSB-EP810145DQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Cytochrome c biogenesis protein CCS1, chloroplastic (CCS1), partial CSB-YP810146DSJ1
CSB-EP810146DSJ1
CSB-BP810146DSJ1
CSB-MP810146DSJ1
CSB-EP810146DSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable pleiotropic drug resistance protein 2 (PDR2), partial CSB-YP810147OFG
CSB-EP810147OFG
CSB-BP810147OFG
CSB-MP810147OFG
CSB-EP810147OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At1g01500 (At1g01500), partial CSB-YP810148DOA1
CSB-EP810148DOA1
CSB-BP810148DOA1
CSB-MP810148DOA1
CSB-EP810148DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized GPI-anchored protein At5g19230 (At5g19230) CSB-YP810149DOA
CSB-EP810149DOA
CSB-BP810149DOA
CSB-MP810149DOA
CSB-EP810149DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin protein ligase RIE1 (RIE1), partial CSB-YP810150DOA1
CSB-EP810150DOA1
CSB-BP810150DOA1
CSB-MP810150DOA1
CSB-EP810150DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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