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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus epidermidis Extracellular matrix-binding protein ebh (ebh), partial CSB-YP810643SLD
CSB-EP810643SLD
CSB-BP810643SLD
CSB-MP810643SLD
CSB-EP810643SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Indole-3-glycerol phosphate synthase (trpC) CSB-YP810644SLD
CSB-EP810644SLD
CSB-BP810644SLD
CSB-MP810644SLD
CSB-EP810644SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA polymerase III polC-type (polC), partial CSB-YP810645SLD
CSB-EP810645SLD
CSB-BP810645SLD
CSB-MP810645SLD
CSB-EP810645SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Ribonuclease 3 (rnc) CSB-YP810646SLD
CSB-EP810646SLD
CSB-BP810646SLD
CSB-MP810646SLD
CSB-EP810646SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Aspartate carbamoyltransferase (pyrB) CSB-YP810647SLD
CSB-EP810647SLD
CSB-BP810647SLD
CSB-MP810647SLD
CSB-EP810647SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Peptide deformylase (def) CSB-YP810648SLD
CSB-EP810648SLD
CSB-BP810648SLD
CSB-MP810648SLD
CSB-EP810648SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable quinol oxidase subunit 2 (qoxA), partial CSB-YP810649SLD1
CSB-EP810649SLD1
CSB-BP810649SLD1
CSB-MP810649SLD1
CSB-EP810649SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP810650SLD
CSB-EP810650SLD
CSB-BP810650SLD
CSB-MP810650SLD
CSB-EP810650SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP810651SLD
CSB-EP810651SLD
CSB-BP810651SLD
CSB-MP810651SLD
CSB-EP810651SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Na (+)/H (+) antiporter subunit E1 (mnhE1), partial CSB-YP810652SLD1
CSB-EP810652SLD1
CSB-BP810652SLD1
CSB-MP810652SLD1
CSB-EP810652SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glyceraldehyde-3-phosphate dehydrogenase 1 (gapA1) CSB-YP810653SLD
CSB-EP810653SLD
CSB-BP810653SLD
CSB-MP810653SLD
CSB-EP810653SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP810654SLD
CSB-EP810654SLD
CSB-BP810654SLD
CSB-MP810654SLD
CSB-EP810654SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative antiporter subunit mnhF2 (mnhF2), partial CSB-YP810655SLD1
CSB-EP810655SLD1
CSB-BP810655SLD1
CSB-MP810655SLD1
CSB-EP810655SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis ATP-dependent Clp protease ATP-binding subunit ClpC (clpC), partial CSB-YP810656SLD
CSB-EP810656SLD
CSB-BP810656SLD
CSB-MP810656SLD
CSB-EP810656SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP810657SLD
CSB-EP810657SLD
CSB-BP810657SLD
CSB-MP810657SLD
CSB-EP810657SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP810658SLD
CSB-EP810658SLD
CSB-BP810658SLD
CSB-MP810658SLD
CSB-EP810658SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Arsenical pump membrane protein (arsB), partial CSB-YP810659SLD
CSB-EP810659SLD
CSB-BP810659SLD
CSB-MP810659SLD
CSB-EP810659SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Chromosomal replication initiator protein DnaA (dnaA) CSB-YP810660SLD
CSB-EP810660SLD
CSB-BP810660SLD
CSB-MP810660SLD
CSB-EP810660SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized protein SE_2353 (SE_2353) CSB-YP810661SLD
CSB-EP810661SLD
CSB-BP810661SLD
CSB-MP810661SLD
CSB-EP810661SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0324 membrane protein SE_2309 (SE_2309), partial CSB-YP810662SLD
CSB-EP810662SLD
CSB-BP810662SLD
CSB-MP810662SLD
CSB-EP810662SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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