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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Chlorobium tepidum Sec-independent protein translocase protein TatA (tatA), partial CSB-YP810473DST
CSB-EP810473DST
CSB-BP810473DST
CSB-MP810473DST
CSB-EP810473DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP810474DST
CSB-EP810474DST
CSB-BP810474DST
CSB-MP810474DST
CSB-EP810474DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum 50S ribosomal protein L21 (rplU) CSB-YP810475DST
CSB-EP810475DST
CSB-BP810475DST
CSB-MP810475DST
CSB-EP810475DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Heat-inducible transcription repressor HrcA (hrcA) CSB-YP810476DST
CSB-EP810476DST
CSB-BP810476DST
CSB-MP810476DST
CSB-EP810476DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Phosphomethylpyrimidine synthase (thiC), partial CSB-YP810477DST
CSB-EP810477DST
CSB-BP810477DST
CSB-MP810477DST
CSB-EP810477DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP810478DST
CSB-EP810478DST
CSB-BP810478DST
CSB-MP810478DST
CSB-EP810478DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP810479DST
CSB-EP810479DST
CSB-BP810479DST
CSB-MP810479DST
CSB-EP810479DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Ferredoxin-1 (CT1261) CSB-YP810480DST
CSB-EP810480DST
CSB-BP810480DST
CSB-MP810480DST
CSB-EP810480DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Dephospho-CoA kinase (coaE) CSB-YP810481DST
CSB-EP810481DST
CSB-BP810481DST
CSB-MP810481DST
CSB-EP810481DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Thiamine-phosphate synthase (thiE) CSB-YP810482DST
CSB-EP810482DST
CSB-BP810482DST
CSB-MP810482DST
CSB-EP810482DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP810483DST1
CSB-EP810483DST1
CSB-BP810483DST1
CSB-MP810483DST1
CSB-EP810483DST1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Maf-like protein CT0974 (maf) CSB-YP810484DST
CSB-EP810484DST
CSB-BP810484DST
CSB-MP810484DST
CSB-EP810484DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP810485DST
CSB-EP810485DST
CSB-BP810485DST
CSB-MP810485DST
CSB-EP810485DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Recombination protein RecR (recR) CSB-YP810486DST
CSB-EP810486DST
CSB-BP810486DST
CSB-MP810486DST
CSB-EP810486DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Ribonuclease Y (rny), partial CSB-YP810487DST
CSB-EP810487DST
CSB-BP810487DST
CSB-MP810487DST
CSB-EP810487DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Quinolinate synthase A (nadA) CSB-YP810488DST
CSB-EP810488DST
CSB-BP810488DST
CSB-MP810488DST
CSB-EP810488DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Histidinol dehydrogenase (hisD) CSB-YP810489DST
CSB-EP810489DST
CSB-BP810489DST
CSB-MP810489DST
CSB-EP810489DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP810490DST
CSB-EP810490DST
CSB-BP810490DST
CSB-MP810490DST
CSB-EP810490DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP810491DST
CSB-EP810491DST
CSB-BP810491DST
CSB-MP810491DST
CSB-EP810491DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP810492DST
CSB-EP810492DST
CSB-BP810492DST
CSB-MP810492DST
CSB-EP810492DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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