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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rickettsia felis Elongation factor Tu (tuf) CSB-YP810513RMT
CSB-EP810513RMT
CSB-BP810513RMT
CSB-MP810513RMT
CSB-EP810513RMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Elongation factor G (fusA), partial CSB-YP810514RNE
CSB-EP810514RNE
CSB-BP810514RNE
CSB-MP810514RNE
CSB-EP810514RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP810515RMV
CSB-EP810515RMV
CSB-BP810515RMV
CSB-MP810515RMV
CSB-EP810515RMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Fumarate hydratase class II (fumC) CSB-YP810516MSZ
CSB-EP810516MSZ
CSB-BP810516MSZ
CSB-MP810516MSZ
CSB-EP810516MSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP810517THN
CSB-EP810517THN
CSB-BP810517THN
CSB-MP810517THN
CSB-EP810517THN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 50S ribosomal protein L33 1 (rpmG1) CSB-YP810518ELW
CSB-EP810518ELW
CSB-BP810518ELW
CSB-MP810518ELW
CSB-EP810518ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens Bacilysin biosynthesis oxidoreductase BacC (bacC) CSB-YP810519BQB
CSB-EP810519BQB
CSB-BP810519BQB
CSB-MP810519BQB
CSB-EP810519BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative bacilysin exporter BacE (bacE), partial CSB-YP810520BRI
CSB-EP810520BRI
CSB-BP810520BRI
CSB-MP810520BRI
CSB-EP810520BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaplasma marginale DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP810521BYV
CSB-EP810521BYV
CSB-BP810521BYV
CSB-MP810521BYV
CSB-EP810521BYV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit 3 (ndhC), partial CSB-YP810522FQA1
CSB-EP810522FQA1
CSB-BP810522FQA1
CSB-MP810522FQA1
CSB-EP810522FQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant K (+)-insensitive pyrophosphate-energized proton pump (hppA), partial CSB-YP810523RDW
CSB-EP810523RDW
CSB-BP810523RDW
CSB-MP810523RDW
CSB-EP810523RDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lechevalieria aerocolonigenes Rebeccamycin O-methyltransferase (rebM) CSB-YP810524LDI
CSB-EP810524LDI
CSB-BP810524LDI
CSB-MP810524LDI
CSB-EP810524LDI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Comamonas testosteroni 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (mhpC) CSB-YP810525CNN
CSB-EP810525CNN
CSB-BP810525CNN
CSB-MP810525CNN
CSB-EP810525CNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP810526BAN
CSB-EP810526BAN
CSB-BP810526BAN
CSB-MP810526BAN
CSB-EP810526BAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP810527PCE
CSB-EP810527PCE
CSB-BP810527PCE
CSB-MP810527PCE
CSB-EP810527PCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE (grpE) CSB-YP810528MQS
CSB-EP810528MQS
CSB-BP810528MQS
CSB-MP810528MQS
CSB-EP810528MQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP810529ABI
CSB-EP810529ABI
CSB-BP810529ABI
CSB-MP810529ABI
CSB-EP810529ABI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Proteasome subunit alpha type-2-B (PAB2) CSB-YP810530DOA
CSB-EP810530DOA
CSB-BP810530DOA
CSB-MP810530DOA
CSB-EP810530DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago truncatula Nodulation receptor kinase (NORK), partial CSB-YP810531MQP
CSB-EP810531MQP
CSB-BP810531MQP
CSB-MP810531MQP
CSB-EP810531MQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana LOB domain-containing protein 15 (LBD15) CSB-YP810532DOA
CSB-EP810532DOA
CSB-BP810532DOA
CSB-MP810532DOA
CSB-EP810532DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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