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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g33811 (At1g33811) CSB-YP810533DOA
CSB-EP810533DOA
CSB-BP810533DOA
CSB-MP810533DOA
CSB-EP810533DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum DNA-directed RNA polymerase 2A (RPOT2-SYL) CSB-YP810534NHE
CSB-EP810534NHE
CSB-BP810534NHE
CSB-MP810534NHE
CSB-EP810534NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable beta-1,4-xylosyltransferase IRX14 (IRX14), partial CSB-YP810535DOA
CSB-EP810535DOA
CSB-BP810535DOA
CSB-MP810535DOA
CSB-EP810535DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein SKIP11 (SKIP11) CSB-YP810536DOA
CSB-EP810536DOA
CSB-BP810536DOA
CSB-MP810536DOA
CSB-EP810536DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 5 [UDP-forming] (CESA5), partial CSB-YP810537DOA
CSB-EP810537DOA
CSB-BP810537DOA
CSB-MP810537DOA
CSB-EP810537DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Inositol oxygenase 1 (MIOX1) CSB-YP810538DOA
CSB-EP810538DOA
CSB-BP810538DOA
CSB-MP810538DOA
CSB-EP810538DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin response factor 1 (ARF1), partial CSB-YP810539DOA
CSB-EP810539DOA
CSB-BP810539DOA
CSB-MP810539DOA
CSB-EP810539DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Homeobox-leucine zipper protein HDG4 (HDG4), partial CSB-YP810540DOA
CSB-EP810540DOA
CSB-BP810540DOA
CSB-MP810540DOA
CSB-EP810540DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At3g23200 (At3g23200), partial CSB-YP810541DOA1
CSB-EP810541DOA1
CSB-BP810541DOA1
CSB-MP810541DOA1
CSB-EP810541DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 3, chloroplastic (RH3), partial CSB-YP810542DOA
CSB-EP810542DOA
CSB-BP810542DOA
CSB-MP810542DOA
CSB-EP810542DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Anamorsin homolog (At5g18400) CSB-YP810543DOA
CSB-EP810543DOA
CSB-BP810543DOA
CSB-MP810543DOA
CSB-EP810543DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable E3 ubiquitin-protein ligase ARI5 (ARI5), partial CSB-YP810544DOA
CSB-EP810544DOA
CSB-BP810544DOA
CSB-MP810544DOA
CSB-EP810544DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATP-dependent DNA helicase Q-like 4A (RECQL4A), partial CSB-YP810545DOA
CSB-EP810545DOA
CSB-BP810545DOA
CSB-MP810545DOA
CSB-EP810545DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Medicago truncatula Auxin transporter-like protein 5 (LAX5), partial CSB-YP810546MQP
CSB-EP810546MQP
CSB-BP810546MQP
CSB-MP810546MQP
CSB-EP810546MQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Iron-sulfur assembly protein IscA-like 3, mitochondrial (At2g36260) CSB-YP810547DOA
CSB-EP810547DOA
CSB-BP810547DOA
CSB-MP810547DOA
CSB-EP810547DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At4g25830 (At4g25830), partial CSB-YP810548DOA1
CSB-EP810548DOA1
CSB-BP810548DOA1
CSB-MP810548DOA1
CSB-EP810548DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative clathrin assembly protein At4g40080 (At4g40080) CSB-YP810549DOA
CSB-EP810549DOA
CSB-BP810549DOA
CSB-MP810549DOA
CSB-EP810549DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S27-3 (RPS27D) CSB-YP810550DOA
CSB-EP810550DOA
CSB-BP810550DOA
CSB-MP810550DOA
CSB-EP810550DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At4g16442 (At4g16442), partial CSB-YP810551DOA1
CSB-EP810551DOA1
CSB-BP810551DOA1
CSB-MP810551DOA1
CSB-EP810551DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor 13 (ERF13) CSB-YP810552DOA
CSB-EP810552DOA
CSB-BP810552DOA
CSB-MP810552DOA
CSB-EP810552DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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