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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus epidermidis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP810663SLD
CSB-EP810663SLD
CSB-BP810663SLD
CSB-MP810663SLD
CSB-EP810663SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP810664SLD
CSB-EP810664SLD
CSB-BP810664SLD
CSB-MP810664SLD
CSB-EP810664SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Holin-like protein CidA (cidA), partial CSB-YP810665SLD1
CSB-EP810665SLD1
CSB-BP810665SLD1
CSB-MP810665SLD1
CSB-EP810665SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA topoisomerase 3 (topB), partial CSB-YP810666SLD
CSB-EP810666SLD
CSB-BP810666SLD
CSB-MP810666SLD
CSB-EP810666SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 50S ribosomal protein L30 (rpmD) CSB-YP810667SLD
CSB-EP810667SLD
CSB-BP810667SLD
CSB-MP810667SLD
CSB-EP810667SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP810668SLD
CSB-EP810668SLD
CSB-BP810668SLD
CSB-MP810668SLD
CSB-EP810668SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Galactose-6-phosphate isomerase subunit lacA (lacA) CSB-YP810669SLD
CSB-EP810669SLD
CSB-BP810669SLD
CSB-MP810669SLD
CSB-EP810669SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP810670SLD
CSB-EP810670SLD
CSB-BP810670SLD
CSB-MP810670SLD
CSB-EP810670SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uracil phosphoribosyltransferase (upp) CSB-YP810671SLD
CSB-EP810671SLD
CSB-BP810671SLD
CSB-MP810671SLD
CSB-EP810671SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0316 protein SE_1595 (SE_1595), partial CSB-YP810672SLD1
CSB-EP810672SLD1
CSB-BP810672SLD1
CSB-MP810672SLD1
CSB-EP810672SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA ligase (ligA), partial CSB-YP810673SLD
CSB-EP810673SLD
CSB-BP810673SLD
CSB-MP810673SLD
CSB-EP810673SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0421 protein SE_1574 (SE_1574), partial CSB-YP810674SLD1
CSB-EP810674SLD1
CSB-BP810674SLD1
CSB-MP810674SLD1
CSB-EP810674SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glutamate-1-semialdehyde 2,1-aminomutase 2 (hemL2) CSB-YP810675SLD
CSB-EP810675SLD
CSB-BP810675SLD
CSB-MP810675SLD
CSB-EP810675SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Protein CrcB homolog 2 (crcB2), partial CSB-YP810676SLD1
CSB-EP810676SLD1
CSB-BP810676SLD1
CSB-MP810676SLD1
CSB-EP810676SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative universal stress protein SE_1385 (SE_1385) CSB-YP810677SLD
CSB-EP810677SLD
CSB-BP810677SLD
CSB-MP810677SLD
CSB-EP810677SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Threonine--tRNA ligase (thrS), partial CSB-YP810678SLD
CSB-EP810678SLD
CSB-BP810678SLD
CSB-MP810678SLD
CSB-EP810678SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP810679SLD
CSB-EP810679SLD
CSB-BP810679SLD
CSB-MP810679SLD
CSB-EP810679SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0473 protein SE_1298 (SE_1298) CSB-YP810680SLD
CSB-EP810680SLD
CSB-BP810680SLD
CSB-MP810680SLD
CSB-EP810680SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable rRNA maturation factor (SE_1257) CSB-YP810681SLD
CSB-EP810681SLD
CSB-BP810681SLD
CSB-MP810681SLD
CSB-EP810681SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Ribonuclease Z (rnz) CSB-YP810682SLD
CSB-EP810682SLD
CSB-BP810682SLD
CSB-MP810682SLD
CSB-EP810682SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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