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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Monosiga brevicollis Flap endonuclease 1 (FEN1) CSB-YP008585MOD
CSB-EP008585MOD
CSB-BP008585MOD
CSB-MP008585MOD
CSB-EP008585MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 6-phosphofructokinase (pfkA) CSB-YP017822BON
CSB-EP017822BON
CSB-BP017822BON
CSB-MP017822BON
CSB-EP017822BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Argininosuccinate synthase (argG) CSB-YP002234BON
CSB-EP002234BON
CSB-BP002234BON
CSB-MP002234BON
CSB-EP002234BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glycine cleavage system H protein (gcvH) CSB-YP009335BON
CSB-EP009335BON
CSB-BP009335BON
CSB-MP009335BON
CSB-EP009335BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582BON
CSB-EP009582BON
CSB-BP009582BON
CSB-MP009582BON
CSB-EP009582BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824BON
CSB-EP005824BON
CSB-BP005824BON
CSB-MP005824BON
CSB-EP005824BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Serine hydroxymethyltransferase (glyA) CSB-YP021274MNY
CSB-EP021274MNY
CSB-BP021274MNY
CSB-MP021274MNY
CSB-EP021274MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens ATP synthase subunit alpha (atpA), partial CSB-YP002344MNY
CSB-EP002344MNY
CSB-BP002344MNY
CSB-MP002344MNY
CSB-EP002344MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Malate dehydrogenase (mdh) CSB-YP013623MNY
CSB-EP013623MNY
CSB-BP013623MNY
CSB-MP013623MNY
CSB-EP013623MNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Peptide chain release factor 1 (prfA) CSB-YP015197DSX
CSB-EP015197DSX
CSB-BP015197DSX
CSB-MP015197DSX
CSB-EP015197DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aurantiacus Translation initiation factor IF-2 (infB), partial CSB-YP015164DSX
CSB-EP015164DSX
CSB-BP015164DSX
CSB-MP015164DSX
CSB-EP015164DSX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum ATP synthase subunit alpha (atpA), partial CSB-YP002344RIH
CSB-EP002344RIH
CSB-BP002344RIH
CSB-MP002344RIH
CSB-EP002344RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Translation initiation factor IF-2 (infB), partial CSB-YP015164RIH
CSB-EP015164RIH
CSB-BP015164RIH
CSB-MP015164RIH
CSB-EP015164RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Renibacterium salmoninarum Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824RIH
CSB-EP005824RIH
CSB-BP005824RIH
CSB-MP005824RIH
CSB-EP005824RIH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio anubis Oocyte-expressed protein homolog (OOEP) CSB-YP016339ERI
CSB-EP016339ERI
CSB-BP016339ERI
CSB-MP016339ERI
CSB-EP016339ERI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrosopumilus maritimus Malate dehydrogenase (mdh) CSB-YP013623NFB
CSB-EP013623NFB
CSB-BP013623NFB
CSB-MP013623NFB
CSB-EP013623NFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658MNQ
CSB-EP001658MNQ
CSB-BP001658MNQ
CSB-MP001658MNQ
CSB-EP001658MNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Acyl carrier protein (acpP) CSB-YP015636BPS
CSB-EP015636BPS
CSB-BP015636BPS
CSB-MP015636BPS
CSB-EP015636BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans ATP synthase subunit b (atpF), partial CSB-YP002358BPS1
CSB-EP002358BPS1
CSB-BP002358BPS1
CSB-MP002358BPS1
CSB-EP002358BPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Herpetosiphon aurantiacus Elongation factor Ts (tsf) CSB-YP025124HSK
CSB-EP025124HSK
CSB-BP025124HSK
CSB-MP025124HSK
CSB-EP025124HSK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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