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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Natranaerobius thermophilus Elongation factor G (fusA), partial CSB-YP009373NFO
CSB-EP009373NFO
CSB-BP009373NFO
CSB-MP009373NFO
CSB-EP009373NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natranaerobius thermophilus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582NFO
CSB-EP009582NFO
CSB-BP009582NFO
CSB-MP009582NFO
CSB-EP009582NFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithodoros coriaceus Defensin CSB-YP006652OEW
CSB-EP006652OEW
CSB-BP006652OEW
CSB-MP006652OEW
CSB-EP006652OEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917FLX
CSB-EP022917FLX
CSB-BP022917FLX
CSB-MP022917FLX
CSB-EP022917FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Peptide deformylase (def) CSB-YP017707LMV
CSB-EP017707LMV
CSB-BP017707LMV
CSB-MP017707LMV
CSB-EP017707LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri Elongation factor Ts (tsf) CSB-YP025124LMV
CSB-EP025124LMV
CSB-BP025124LMV
CSB-MP025124LMV
CSB-EP025124LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus reuteri tRNA pseudouridine synthase A (truA) CSB-YP019086LMV
CSB-EP019086LMV
CSB-BP019086LMV
CSB-MP019086LMV
CSB-EP019086LMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum ATP synthase subunit b (atpF), partial CSB-YP002358LMO1
CSB-EP002358LMO1
CSB-BP002358LMO1
CSB-MP002358LMO1
CSB-EP002358LMO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus fermentum Galactokinase (galK) CSB-YP009198LMO
CSB-EP009198LMO
CSB-BP009198LMO
CSB-MP009198LMO
CSB-EP009198LMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582MVS
CSB-EP009582MVS
CSB-BP009582MVS
CSB-MP009582MVS
CSB-EP009582MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP017614MVS
CSB-EP017614MVS
CSB-BP017614MVS
CSB-MP017614MVS
CSB-EP017614MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Peptide chain release factor 1 (prfA) CSB-YP015197MVS
CSB-EP015197MVS
CSB-BP015197MVS
CSB-MP015197MVS
CSB-EP015197MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Argininosuccinate synthase (argG) CSB-YP002234MVS
CSB-EP002234MVS
CSB-BP002234MVS
CSB-MP002234MVS
CSB-EP002234MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Malate dehydrogenase (mdh) CSB-YP013623MVS
CSB-EP013623MVS
CSB-BP013623MVS
CSB-MP013623MVS
CSB-EP013623MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Elongation factor Tu (tuf) CSB-YP025342MVS
CSB-EP025342MVS
CSB-BP025342MVS
CSB-MP025342MVS
CSB-EP025342MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917AWO
CSB-EP022917AWO
CSB-BP022917AWO
CSB-MP022917AWO
CSB-EP022917AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii ATP synthase subunit delta (atpH) CSB-YP002355AWO
CSB-EP002355AWO
CSB-BP002355AWO
CSB-MP002355AWO
CSB-EP002355AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Triosephosphate isomerase (tpiA) CSB-YP024102AWO
CSB-EP024102AWO
CSB-BP024102AWO
CSB-MP024102AWO
CSB-EP024102AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Enolase-phosphatase E1 (mtnC) CSB-YP007674XBN
CSB-EP007674XBN
CSB-BP007674XBN
CSB-MP007674XBN
CSB-EP007674XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Triosephosphate isomerase (tpiA) CSB-YP024102XBN
CSB-EP024102XBN
CSB-BP024102XBN
CSB-MP024102XBN
CSB-EP024102XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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