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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 Glycine cleavage system H protein (gcvH) CSB-YP009335SYZ
CSB-EP009335SYZ
CSB-BP009335SYZ
CSB-MP009335SYZ
CSB-EP009335SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 N-acetylneuraminate lyase (nanA) CSB-YP015994SYZ
CSB-EP015994SYZ
CSB-BP015994SYZ
CSB-MP015994SYZ
CSB-EP015994SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SYZ
CSB-EP015150SYZ
CSB-BP015150SYZ
CSB-MP015150SYZ
CSB-EP015150SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Elongation factor Ts (tsf) CSB-YP025124SYZ
CSB-EP025124SYZ
CSB-BP025124SYZ
CSB-MP025124SYZ
CSB-EP025124SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203RJI
CSB-EP001203RJI
CSB-BP001203RJI
CSB-MP001203RJI
CSB-EP001203RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Malate dehydrogenase (mdh) CSB-YP013623RJI
CSB-EP013623RJI
CSB-BP013623RJI
CSB-MP013623RJI
CSB-EP013623RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Transaldolase (tal) CSB-YP023112RJI
CSB-EP023112RJI
CSB-BP023112RJI
CSB-MP023112RJI
CSB-EP023112RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii ATP synthase subunit b (atpF), partial CSB-YP002358RJI1
CSB-EP002358RJI1
CSB-BP002358RJI1
CSB-MP002358RJI1
CSB-EP002358RJI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917AZF
CSB-EP022917AZF
CSB-BP022917AZF
CSB-MP022917AZF
CSB-EP022917AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Peptide chain release factor 1 (prfA) CSB-YP015197HUZ
CSB-EP015197HUZ
CSB-BP015197HUZ
CSB-MP015197HUZ
CSB-EP015197HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribonuclease 3 (rnc) CSB-YP019809HUZ
CSB-EP019809HUZ
CSB-BP019809HUZ
CSB-MP019809HUZ
CSB-EP019809HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Elongation factor G (fusA), partial CSB-YP009373HUZ
CSB-EP009373HUZ
CSB-BP009373HUZ
CSB-MP009373HUZ
CSB-EP009373HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Elongation factor Tu (tuf) CSB-YP025342HUZ
CSB-EP025342HUZ
CSB-BP025342HUZ
CSB-MP025342HUZ
CSB-EP025342HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033HUZ
CSB-EP018033HUZ
CSB-BP018033HUZ
CSB-MP018033HUZ
CSB-EP018033HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Elongation factor Ts (tsf) CSB-YP025124HUZ
CSB-EP025124HUZ
CSB-BP025124HUZ
CSB-MP025124HUZ
CSB-EP025124HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum GTPase Era (era) CSB-YP007759DUF
CSB-EP007759DUF
CSB-BP007759DUF
CSB-MP007759DUF
CSB-EP007759DUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurihydrogenibium sp. ATP synthase subunit a (atpB), partial CSB-YP015070FPP1
CSB-EP015070FPP1
CSB-BP015070FPP1
CSB-MP015070FPP1
CSB-EP015070FPP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451ENB
CSB-EP015451ENB
CSB-BP015451ENB
CSB-MP015451ENB
CSB-EP015451ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis ATP synthase subunit a (atpB), partial CSB-YP015070ENB1
CSB-EP015070ENB1
CSB-BP015070ENB1
CSB-MP015070ENB1
CSB-EP015070ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Ribosome-recycling factor (frr) CSB-YP014941ENB
CSB-EP014941ENB
CSB-BP014941ENB
CSB-MP014941ENB
CSB-EP014941ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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