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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii ATP synthase subunit b (atpF), partial CSB-YP002358DXN1
CSB-EP002358DXN1
CSB-BP002358DXN1
CSB-MP002358DXN1
CSB-EP002358DXN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans ATP synthase subunit alpha (atpA), partial CSB-YP002344OED
CSB-EP002344OED
CSB-BP002344OED
CSB-MP002344OED
CSB-EP002344OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Lipoyl synthase (lipA) CSB-YP012927OED
CSB-EP012927OED
CSB-BP012927OED
CSB-MP012927OED
CSB-EP012927OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans GTPase Era (era) CSB-YP007759OED
CSB-EP007759OED
CSB-BP007759OED
CSB-MP007759OED
CSB-EP007759OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Porphobilinogen deaminase (hemC) CSB-YP010524HUY
CSB-EP010524HUY
CSB-BP010524HUY
CSB-MP010524HUY
CSB-EP010524HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146HUY
CSB-EP019146HUY
CSB-BP019146HUY
CSB-MP019146HUY
CSB-EP019146HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203HUY
CSB-EP001203HUY
CSB-BP001203HUY
CSB-MP001203HUY
CSB-EP001203HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582HUY
CSB-EP009582HUY
CSB-BP009582HUY
CSB-MP009582HUY
CSB-EP009582HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP synthase subunit b (atpF), partial CSB-YP002358HUY1
CSB-EP002358HUY1
CSB-BP002358HUY1
CSB-MP002358HUY1
CSB-EP002358HUY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033HUY
CSB-EP018033HUY
CSB-BP018033HUY
CSB-MP018033HUY
CSB-EP018033HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Elongation factor Ts (tsf) CSB-YP025124HUY
CSB-EP025124HUY
CSB-BP025124HUY
CSB-MP025124HUY
CSB-EP025124HUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae Histone H2A deubiquitinase MYSM1 (MYSM1), partial CSB-YP015382BVY
CSB-EP015382BVY
CSB-BP015382BVY
CSB-MP015382BVY
CSB-EP015382BVY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium marneffei Structure-specific endonuclease subunit slx1 (slx1) CSB-YP009442ETM
CSB-EP009442ETM
CSB-BP009442ETM
CSB-MP009442ETM
CSB-EP009442ETM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Pleckstrin homology domain-containing family A member 7 (plekha7), partial CSB-YP018161DIL
CSB-EP018161DIL
CSB-BP018161DIL
CSB-MP018161DIL
CSB-EP018161DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Ubiquitin-related modifier 1 homolog (URM1) CSB-YP025676ZAX
CSB-EP025676ZAX
CSB-BP025676ZAX
CSB-MP025676ZAX
CSB-EP025676ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Methylthioribose-1-phosphate isomerase (IDI2) CSB-YP014799ZAX
CSB-EP014799ZAX
CSB-BP014799ZAX
CSB-MP014799ZAX
CSB-EP014799ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform (ppp2r2b) CSB-YP018565DQG
CSB-EP018565DQG
CSB-BP018565DQG
CSB-MP018565DQG
CSB-EP018565DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP019086DPP
CSB-EP019086DPP
CSB-BP019086DPP
CSB-MP019086DPP
CSB-EP019086DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoserine aminotransferase (serC) CSB-YP018838DPP
CSB-EP018838DPP
CSB-BP018838DPP
CSB-MP018838DPP
CSB-EP018838DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Ts (tsf) CSB-YP025124DPP
CSB-EP025124DPP
CSB-BP025124DPP
CSB-MP025124DPP
CSB-EP025124DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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