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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila mojavensis Ubiquitin-conjugating enzyme E2 S (GI11085) CSB-YP025477DLY
CSB-EP025477DLY
CSB-BP025477DLY
CSB-MP025477DLY
CSB-EP025477DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Eukaryotic translation initiation factor 3 subunit C (eIF3-S8), partial CSB-YP007531DMP
CSB-EP007531DMP
CSB-BP007531DMP
CSB-MP007531DMP
CSB-EP007531DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Pescadillo homolog (GJ14807), partial CSB-YP017791DMP
CSB-EP017791DMP
CSB-BP017791DMP
CSB-MP017791DMP
CSB-EP017791DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis AF4/FMR2 family member 4 (lilli), partial CSB-YP001415DMP
CSB-EP001415DMP
CSB-BP001415DMP
CSB-MP001415DMP
CSB-EP001415DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Methylthioribose-1-phosphate isomerase (GJ22917) CSB-YP014799DMP
CSB-EP014799DMP
CSB-BP014799DMP
CSB-MP014799DMP
CSB-EP014799DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Probable methylthioribulose-1-phosphate dehydratase (GJ19387) CSB-YP001906DMP
CSB-EP001906DMP
CSB-BP001906DMP
CSB-MP001906DMP
CSB-EP001906DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis MAU2 chromatid cohesion factor homolog (GJ10962), partial CSB-YP012213DMP
CSB-EP012213DMP
CSB-BP012213DMP
CSB-MP012213DMP
CSB-EP012213DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Zinc finger CCCH-type with G patch domain-containing protein (GJ17921), partial CSB-YP026480DMP
CSB-EP026480DMP
CSB-BP026480DMP
CSB-MP026480DMP
CSB-EP026480DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis 40S ribosomal protein SA (sta) CSB-YP020485DMP
CSB-EP020485DMP
CSB-BP020485DMP
CSB-MP020485DMP
CSB-EP020485DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Protein arginine N-methyltransferase 7 (Art7), partial CSB-YP018736DMQ
CSB-EP018736DMQ
CSB-BP018736DMQ
CSB-MP018736DMQ
CSB-EP018736DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Ubiquitin-conjugating enzyme E2 S (GK16201) CSB-YP025477DMQ
CSB-EP025477DMQ
CSB-BP025477DMQ
CSB-MP025477DMQ
CSB-EP025477DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Nuclear cap-binding protein subunit 2 (Cbp20) CSB-YP015521DMQ
CSB-EP015521DMQ
CSB-BP015521DMQ
CSB-MP015521DMQ
CSB-EP015521DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Ubiquitin-like modifier-activating enzyme 5 (GK10218) CSB-YP025415DMQ
CSB-EP025415DMQ
CSB-BP025415DMQ
CSB-MP025415DMQ
CSB-EP025415DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Probable methylthioribulose-1-phosphate dehydratase (GK25216) CSB-YP001906DMQ
CSB-EP001906DMQ
CSB-BP001906DMQ
CSB-MP001906DMQ
CSB-EP001906DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni 40S ribosomal protein S3a (RpS3A) CSB-YP020444DMQ
CSB-EP020444DMQ
CSB-BP020444DMQ
CSB-MP020444DMQ
CSB-EP020444DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Cytoplasmic tRNA 2-thiolation protein 1 (GK22963) CSB-YP002426DMQ
CSB-EP002426DMQ
CSB-BP002426DMQ
CSB-MP002426DMQ
CSB-EP002426DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Adenylyltransferase and sulfurtransferase MOCS3 (GE18783) CSB-YP014707DMR
CSB-EP014707DMR
CSB-BP014707DMR
CSB-MP014707DMR
CSB-EP014707DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit H (eIF-3p40) CSB-YP007537DMR
CSB-EP007537DMR
CSB-BP007537DMR
CSB-MP007537DMR
CSB-EP007537DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Probable cytosolic iron-sulfur protein assembly protein Ciao1 (Ciao1) CSB-YP005423DMR
CSB-EP005423DMR
CSB-BP005423DMR
CSB-MP005423DMR
CSB-EP005423DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit E (eIF3-S6) CSB-YP007534DMR
CSB-EP007534DMR
CSB-BP007534DMR
CSB-MP007534DMR
CSB-EP007534DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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