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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Salmonella enteritidis PT4 Glycogen debranching enzyme (glgX), partial CSB-YP001446SWO
CSB-EP001446SWO
CSB-BP001446SWO
CSB-MP001446SWO
CSB-EP001446SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Ribosome-recycling factor (frr) CSB-YP014941SWO
CSB-EP014941SWO
CSB-BP014941SWO
CSB-MP014941SWO
CSB-EP014941SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Glucokinase (glk) CSB-YP009319SWO
CSB-EP009319SWO
CSB-BP009319SWO
CSB-MP009319SWO
CSB-EP009319SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Urocanate hydratase (hutU), partial CSB-YP025677SWP
CSB-EP025677SWP
CSB-BP025677SWP
CSB-MP025677SWP
CSB-EP025677SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Thymidylate kinase (tmk) CSB-YP007226SWP
CSB-EP007226SWP
CSB-BP007226SWP
CSB-MP007226SWP
CSB-EP007226SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Nucleoside diphosphate kinase (ndk) CSB-YP015889SWP
CSB-EP015889SWP
CSB-BP015889SWP
CSB-MP015889SWP
CSB-EP015889SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum 6-phosphofructokinase (pfkA) CSB-YP017822SWP
CSB-EP017822SWP
CSB-BP017822SWP
CSB-MP017822SWP
CSB-EP017822SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum Elongation factor G (fusA), partial CSB-YP009373SWP
CSB-EP009373SWP
CSB-BP009373SWP
CSB-MP009373SWP
CSB-EP009373SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella gallinarum S-adenosylmethionine decarboxylase proenzyme (speD) CSB-YP001658SWP
CSB-EP001658SWP
CSB-BP001658SWP
CSB-MP001658SWP
CSB-EP001658SWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Eukaryotic translation initiation factor 3 subunit H (eif3h) CSB-YP007537SWI
CSB-EP007537SWI
CSB-BP007537SWI
CSB-MP007537SWI
CSB-EP007537SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii Methionyl-tRNA formyltransferase (fmt) CSB-YP015150BUH
CSB-EP015150BUH
CSB-BP015150BUH
CSB-MP015150BUH
CSB-EP015150BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia duttonii GTPase Era (era) CSB-YP007759BUH
CSB-EP007759BUH
CSB-BP007759BUH
CSB-MP007759BUH
CSB-EP007759BUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Eukaryotic translation initiation factor 3 subunit A (TIF32), partial CSB-YP007543DIS
CSB-EP007543DIS
CSB-BP007543DIS
CSB-MP007543DIS
CSB-EP007543DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Structure-specific endonuclease subunit SLX1 (SLX1) CSB-YP009442SVH
CSB-EP009442SVH
CSB-BP009442SVH
CSB-MP009442SVH
CSB-EP009442SVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Protein SMG9 (smg9), partial CSB-YP003476SWI
CSB-EP003476SWI
CSB-BP003476SWI
CSB-MP003476SWI
CSB-EP003476SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Actin-related protein 8 (actr8), partial CSB-YP001254SWI
CSB-EP001254SWI
CSB-BP001254SWI
CSB-MP001254SWI
CSB-EP001254SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Tetraspanin-9 (tspan9), partial CSB-YP025167SWI1
CSB-EP025167SWI1
CSB-BP025167SWI1
CSB-MP025167SWI1
CSB-EP025167SWI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar F-box/LRR-repeat protein 15 (fbxl15) CSB-YP008466SWI
CSB-EP008466SWI
CSB-BP008466SWI
CSB-MP008466SWI
CSB-EP008466SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Tumor necrosis factor alpha-induced protein 8-like protein 2 (tnfaip8l2) CSB-YP023962SWI
CSB-EP023962SWI
CSB-BP023962SWI
CSB-MP023962SWI
CSB-EP023962SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar Prostamide/prostaglandin F synthase (fam213b) CSB-YP003630SWI
CSB-EP003630SWI
CSB-BP003630SWI
CSB-MP003630SWI
CSB-EP003630SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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