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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arthrobacter chlorophenolicus ATP synthase subunit alpha (atpA), partial CSB-YP002344DOG
CSB-EP002344DOG
CSB-BP002344DOG
CSB-MP002344DOG
CSB-EP002344DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus Elongation factor G (fusA), partial CSB-YP009373DOG
CSB-EP009373DOG
CSB-BP009373DOG
CSB-MP009373DOG
CSB-EP009373DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. ATP synthase subunit beta (atpD) CSB-YP002350EPV
CSB-EP002350EPV
CSB-BP002350EPV
CSB-MP002350EPV
CSB-EP002350EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Serine hydroxymethyltransferase (glyA) CSB-YP021274EPV
CSB-EP021274EPV
CSB-BP021274EPV
CSB-MP021274EPV
CSB-EP021274EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Argininosuccinate synthase (argG) CSB-YP002234EPV
CSB-EP002234EPV
CSB-BP002234EPV
CSB-MP002234EPV
CSB-EP002234EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Thymidylate kinase (tmk) CSB-YP007226EPV
CSB-EP007226EPV
CSB-BP007226EPV
CSB-MP007226EPV
CSB-EP007226EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Elongation factor G (fusA), partial CSB-YP009373DUL
CSB-EP009373DUL
CSB-BP009373DUL
CSB-MP009373DUL
CSB-EP009373DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033DUL
CSB-EP018033DUL
CSB-BP018033DUL
CSB-MP018033DUL
CSB-EP018033DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717EZY
CSB-EP009717EZY
CSB-BP009717EZY
CSB-MP009717EZY
CSB-EP009717EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Thymidylate synthase (thyA) CSB-YP025393EZY
CSB-EP025393EZY
CSB-BP025393EZY
CSB-MP025393EZY
CSB-EP025393EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033EZY
CSB-EP018033EZY
CSB-BP018033EZY
CSB-MP018033EZY
CSB-EP018033EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Urocanate hydratase (hutU), partial CSB-YP025677EZY
CSB-EP025677EZY
CSB-BP025677EZY
CSB-MP025677EZY
CSB-EP025677EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa ATP synthase subunit a (atpB), partial CSB-YP015070EZY1
CSB-EP015070EZY1
CSB-BP015070EZY1
CSB-MP015070EZY1
CSB-EP015070EZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917VFH
CSB-EP022917VFH
CSB-BP022917VFH
CSB-MP022917VFH
CSB-EP022917VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Translation initiation factor IF-2 (infB), partial CSB-YP015164VFH
CSB-EP015164VFH
CSB-BP015164VFH
CSB-MP015164VFH
CSB-EP015164VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Lipoyl synthase (lipA) CSB-YP012927VFH
CSB-EP012927VFH
CSB-BP012927VFH
CSB-MP012927VFH
CSB-EP012927VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415VFH
CSB-EP015415VFH
CSB-BP015415VFH
CSB-MP015415VFH
CSB-EP015415VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Glycine cleavage system H protein (gcvH) CSB-YP009335VFH
CSB-EP009335VFH
CSB-BP009335VFH
CSB-MP009335VFH
CSB-EP009335VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein ripply3 (dscr6) CSB-YP007198XBE
CSB-EP007198XBE
CSB-BP007198XBE
CSB-MP007198XBE
CSB-EP007198XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutaredoxin-1 (GRX1) CSB-YP009521GGK
CSB-EP009521GGK
CSB-BP009521GGK
CSB-MP009521GGK
CSB-EP009521GGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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