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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O81 ATP synthase subunit delta (atpH) CSB-YP002355EOP
CSB-EP002355EOP
CSB-BP002355EOP
CSB-MP002355EOP
CSB-EP002355EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 3-ketoacyl-CoA thiolase (fadI) CSB-YP001117EOG
CSB-EP001117EOG
CSB-BP001117EOG
CSB-MP001117EOG
CSB-EP001117EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Thymidylate synthase (thyA) CSB-YP025393EOG
CSB-EP025393EOG
CSB-BP025393EOG
CSB-MP025393EOG
CSB-EP025393EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Glycine cleavage system H protein (gcvH) CSB-YP009335EOG
CSB-EP009335EOG
CSB-BP009335EOG
CSB-MP009335EOG
CSB-EP009335EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Beta-galactosidase (lacZ), partial CSB-YP009476EOG
CSB-EP009476EOG
CSB-BP009476EOG
CSB-MP009476EOG
CSB-EP009476EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Galactokinase (galK) CSB-YP009198EOG
CSB-EP009198EOG
CSB-BP009198EOG
CSB-MP009198EOG
CSB-EP009198EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Glycogen debranching enzyme (glgX) CSB-YP001446EOG
CSB-EP001446EOG
CSB-BP001446EOG
CSB-MP001446EOG
CSB-EP001446EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 ATP synthase subunit beta (atpD) CSB-YP002350EOG
CSB-EP002350EOG
CSB-BP002350EOG
CSB-MP002350EOG
CSB-EP002350EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Porphobilinogen deaminase (hemC) CSB-YP010524EOG
CSB-EP010524EOG
CSB-BP010524EOG
CSB-MP010524EOG
CSB-EP010524EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 6-phosphofructokinase (pfkA) CSB-YP017822EOG
CSB-EP017822EOG
CSB-BP017822EOG
CSB-MP017822EOG
CSB-EP017822EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 tRNA dimethylallyltransferase (miaA) CSB-YP024529EOG
CSB-EP024529EOG
CSB-BP024529EOG
CSB-MP024529EOG
CSB-EP024529EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Lipoyl synthase (lipA) CSB-YP012927EON
CSB-EP012927EON
CSB-BP012927EON
CSB-MP012927EON
CSB-EP012927EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Glycogen debranching enzyme (glgX) CSB-YP001446EON
CSB-EP001446EON
CSB-BP001446EON
CSB-MP001446EON
CSB-EP001446EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 ATP synthase subunit alpha (atpA) CSB-YP002344EON
CSB-EP002344EON
CSB-BP002344EON
CSB-MP002344EON
CSB-EP002344EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033EON
CSB-EP018033EON
CSB-BP018033EON
CSB-MP018033EON
CSB-EP018033EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O7:K1 Thymidylate synthase (thyA) CSB-YP025393EON
CSB-EP025393EON
CSB-BP025393EON
CSB-MP025393EON
CSB-EP025393EON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ixodes scapularis U1 small nuclear ribonucleoprotein C (ISCW020627) CSB-YP022331INM
CSB-EP022331INM
CSB-BP022331INM
CSB-MP022331INM
CSB-EP022331INM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Glucokinase (glk) CSB-YP009319EOB
CSB-EP009319EOB
CSB-BP009319EOB
CSB-MP009319EOB
CSB-EP009319EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Thymidylate synthase (thyA) CSB-YP025393EOB
CSB-EP025393EOB
CSB-BP025393EOB
CSB-MP025393EOB
CSB-EP025393EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493EOB
CSB-EP009493EOB
CSB-BP009493EOB
CSB-MP009493EOB
CSB-EP009493EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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