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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Pseudomonas aeruginosa Lipoyl synthase (lipA) CSB-YP012927EZY
CSB-EP012927EZY
CSB-BP012927EZY
CSB-MP012927EZY
CSB-EP012927EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Malate dehydrogenase (mdh) CSB-YP013623VFH
CSB-EP013623VFH
CSB-BP013623VFH
CSB-MP013623VFH
CSB-EP013623VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterocytozoon bieneusi Flap endonuclease 1 (FEN1) CSB-YP008585EMA
CSB-EP008585EMA
CSB-BP008585EMA
CSB-MP008585EMA
CSB-EP008585EMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica TATA box-binding protein-associated factor RNA polymerase I subunit B (OsI_19584), partial CSB-YP023084OFF
CSB-EP023084OFF
CSB-BP023084OFF
CSB-MP023084OFF
CSB-EP023084OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Inosine triphosphate pyrophosphatase (OsI_33887) CSB-YP011907OFF
CSB-EP011907OFF
CSB-BP011907OFF
CSB-MP011907OFF
CSB-EP011907OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Enolase-phosphatase E1 (mtnC) CSB-YP007674SYW
CSB-EP007674SYW
CSB-BP007674SYW
CSB-MP007674SYW
CSB-EP007674SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SYW
CSB-EP015150SYW
CSB-BP015150SYW
CSB-MP015150SYW
CSB-EP015150SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans tRNA dimethylallyltransferase (miaA) CSB-YP024529SYW
CSB-EP024529SYW
CSB-BP024529SYW
CSB-MP024529SYW
CSB-EP024529SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans tRNA pseudouridine synthase A (truA) CSB-YP019086SYW
CSB-EP019086SYW
CSB-BP019086SYW
CSB-MP019086SYW
CSB-EP019086SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Elongation factor Ts (tsf) CSB-YP025124SYW
CSB-EP025124SYW
CSB-BP025124SYW
CSB-MP025124SYW
CSB-EP025124SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Ribonuclease 3 (rnc) CSB-YP019809SYW
CSB-EP019809SYW
CSB-BP019809SYW
CSB-MP019809SYW
CSB-EP019809SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146HUE
CSB-EP019146HUE
CSB-BP019146HUE
CSB-MP019146HUE
CSB-EP019146HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii Thymidylate kinase (tmk) CSB-YP007226HUE
CSB-EP007226HUE
CSB-BP007226HUE
CSB-MP007226HUE
CSB-EP007226HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii Elongation factor G (fusA), partial CSB-YP009373HUE
CSB-EP009373HUE
CSB-BP009373HUE
CSB-MP009373HUE
CSB-EP009373HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Ribonuclease 3 (rnc) CSB-YP019809BXA
CSB-EP019809BXA
CSB-BP019809BXA
CSB-MP019809BXA
CSB-EP019809BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum tRNA dimethylallyltransferase (miaA) CSB-YP024529BXA
CSB-EP024529BXA
CSB-BP024529BXA
CSB-MP024529BXA
CSB-EP024529BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Elongation factor Ts (tsf) CSB-YP025124BWY
CSB-EP025124BWY
CSB-BP025124BWY
CSB-MP025124BWY
CSB-EP025124BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum GTPase Era (era) CSB-YP007759BWY
CSB-EP007759BWY
CSB-BP007759BWY
CSB-MP007759BWY
CSB-EP007759BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 6-phosphofructokinase (pfkA) CSB-YP017822BWY
CSB-EP017822BWY
CSB-BP017822BWY
CSB-MP017822BWY
CSB-EP017822BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a Thymidylate kinase (tmk) CSB-YP007226LPW
CSB-EP007226LPW
CSB-BP007226LPW
CSB-MP007226LPW
CSB-EP007226LPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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