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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O127:H6 Xaa-Pro dipeptidase (pepQ) CSB-YP017784EOB
CSB-EP017784EOB
CSB-BP017784EOB
CSB-MP017784EOB
CSB-EP017784EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 tRNA dimethylallyltransferase (miaA) CSB-YP024529EOB
CSB-EP024529EOB
CSB-BP024529EOB
CSB-MP024529EOB
CSB-EP024529EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa NAD-dependent malic enzyme (maeA), partial CSB-YP013633EZY
CSB-EP013633EZY
CSB-BP013633EZY
CSB-MP013633EZY
CSB-EP013633EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582EZY
CSB-EP009582EZY
CSB-BP009582EZY
CSB-MP009582EZY
CSB-EP009582EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium chloromethanicum Nucleoside diphosphate kinase (ndk) CSB-YP015889MSY
CSB-EP015889MSY
CSB-BP015889MSY
CSB-MP015889MSY
CSB-EP015889MSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli L-threonine 3-dehydrogenase (tdh) CSB-YP023350ENM
CSB-EP023350ENM
CSB-BP023350ENM
CSB-MP023350ENM
CSB-EP023350ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 6-phosphofructokinase (pfkA) CSB-YP017822ENM
CSB-EP017822ENM
CSB-BP017822ENM
CSB-MP017822ENM
CSB-EP017822ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917ENM
CSB-EP022917ENM
CSB-BP022917ENM
CSB-MP022917ENM
CSB-EP022917ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucose-6-phosphate isomerase (pgi) CSB-YP009717ENM
CSB-EP009717ENM
CSB-BP009717ENM
CSB-MP009717ENM
CSB-EP009717ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA pseudouridine synthase A (truA) CSB-YP019086ENM
CSB-EP019086ENM
CSB-BP019086ENM
CSB-MP019086ENM
CSB-EP019086ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Serine hydroxymethyltransferase (glyA) CSB-YP021274ENM
CSB-EP021274ENM
CSB-BP021274ENM
CSB-MP021274ENM
CSB-EP021274ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493ENM
CSB-EP009493ENM
CSB-BP009493ENM
CSB-MP009493ENM
CSB-EP009493ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome-recycling factor (frr) CSB-YP014941ENM
CSB-EP014941ENM
CSB-BP014941ENM
CSB-MP014941ENM
CSB-EP014941ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetylneuraminate lyase (nanA) CSB-YP015994ENM
CSB-EP015994ENM
CSB-BP015994ENM
CSB-MP015994ENM
CSB-EP015994ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Methionyl-tRNA formyltransferase (fmt) CSB-YP015150ENM
CSB-EP015150ENM
CSB-BP015150ENM
CSB-MP015150ENM
CSB-EP015150ENM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii ATP synthase subunit a (atpB), partial CSB-YP015070EOR1
CSB-EP015070EOR1
CSB-BP015070EOR1
CSB-MP015070EOR1
CSB-EP015070EOR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717EOR
CSB-EP009717EOR
CSB-BP009717EOR
CSB-MP009717EOR
CSB-EP009717EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii NAD-dependent malic enzyme (maeA), partial CSB-YP013633EOR
CSB-EP013633EOR
CSB-BP013633EOR
CSB-MP013633EOR
CSB-EP013633EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Elongation factor G (fusA), partial CSB-YP009373EOR
CSB-EP009373EOR
CSB-BP009373EOR
CSB-MP009373EOR
CSB-EP009373EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia fergusonii Thymidylate kinase (tmk) CSB-YP007226EOR
CSB-EP007226EOR
CSB-BP007226EOR
CSB-MP007226EOR
CSB-EP007226EOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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