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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodococcus opacus ATP synthase subunit beta (atpD) CSB-YP002350RLN
CSB-EP002350RLN
CSB-BP002350RLN
CSB-MP002350RLN
CSB-EP002350RLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus opacus Peptide deformylase (def) CSB-YP017707RLN
CSB-EP017707RLN
CSB-BP017707RLN
CSB-MP017707RLN
CSB-EP017707RLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Osmerus mordax Probable methylthioribulose-1-phosphate dehydratase (apip) CSB-YP001906OFM
CSB-EP001906OFM
CSB-BP001906OFM
CSB-MP001906OFM
CSB-EP001906OFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Osmerus mordax Flap endonuclease 1 (fen1) CSB-YP008585OFM
CSB-EP008585OFM
CSB-BP008585OFM
CSB-MP008585OFM
CSB-EP008585OFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Esox lucius Toll-interacting protein (tollip) CSB-YP024042EOU
CSB-EP024042EOU
CSB-BP024042EOU
CSB-MP024042EOU
CSB-EP024042EOU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lithobates catesbeiana 3-hydroxybutyrate dehydrogenase type 2 (bdh2) CSB-YP002649LQA
CSB-EP002649LQA
CSB-BP002649LQA
CSB-MP002649LQA
CSB-EP002649LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribosome-recycling factor (frr) CSB-YP014941FMX
CSB-EP014941FMX
CSB-BP014941FMX
CSB-MP014941FMX
CSB-EP014941FMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae GTPase Era (era) CSB-YP007759FMX
CSB-EP007759FMX
CSB-BP007759FMX
CSB-MP007759FMX
CSB-EP007759FMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Serine hydroxymethyltransferase (glyA) CSB-YP021274FMX
CSB-EP021274FMX
CSB-BP021274FMX
CSB-MP021274FMX
CSB-EP021274FMX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Translation initiation factor IF-3 (infC) CSB-YP015166FNB
CSB-EP015166FNB
CSB-BP015166FNB
CSB-MP015166FNB
CSB-EP015166FNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Ribonuclease 3 (rnc) CSB-YP019809FNB
CSB-EP019809FNB
CSB-BP019809FNB
CSB-MP019809FNB
CSB-EP019809FNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582FNB
CSB-EP009582FNB
CSB-BP009582FNB
CSB-MP009582FNB
CSB-EP009582FNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP synthase subunit beta (atpD) CSB-YP002350FNB
CSB-EP002350FNB
CSB-BP002350FNB
CSB-MP002350FNB
CSB-EP002350FNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA pseudouridine synthase A (truA) CSB-YP019086FNB
CSB-EP019086FNB
CSB-BP019086FNB
CSB-MP019086FNB
CSB-EP019086FNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Elongation factor G (fusA), partial CSB-YP009373FND
CSB-EP009373FND
CSB-BP009373FND
CSB-MP009373FND
CSB-EP009373FND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae tRNA dimethylallyltransferase (miaA) CSB-YP024529FND
CSB-EP024529FND
CSB-BP024529FND
CSB-MP024529FND
CSB-EP024529FND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laribacter hongkongensis ATP synthase subunit beta (atpD) CSB-YP002350LNO
CSB-EP002350LNO
CSB-BP002350LNO
CSB-MP002350LNO
CSB-EP002350LNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laribacter hongkongensis Ribosome-recycling factor (frr) CSB-YP014941LNO
CSB-EP014941LNO
CSB-BP014941LNO
CSB-MP014941LNO
CSB-EP014941LNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laribacter hongkongensis Nucleoside diphosphate kinase (ndk) CSB-YP015889LNO
CSB-EP015889LNO
CSB-BP015889LNO
CSB-MP015889LNO
CSB-EP015889LNO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azotobacter vinelandii Cysteine desulfurase (iscS) CSB-YP015769DPY
CSB-EP015769DPY
CSB-BP015769DPY
CSB-MP015769DPY
CSB-EP015769DPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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