Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium leprae Translation initiation factor IF-2 (infB), partial CSB-YP015164MVO
CSB-EP015164MVO
CSB-BP015164MVO
CSB-MP015164MVO
CSB-EP015164MVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium leprae Elongation factor Tu (tuf) CSB-YP025342MVO
CSB-EP025342MVO
CSB-BP025342MVO
CSB-MP025342MVO
CSB-EP025342MVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium leprae Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033MVO
CSB-EP018033MVO
CSB-BP018033MVO
CSB-MP018033MVO
CSB-EP018033MVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146FLK
CSB-EP019146FLK
CSB-BP019146FLK
CSB-MP019146FLK
CSB-EP019146FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Translation initiation factor IF-2 (infB), partial CSB-YP015164FLK
CSB-EP015164FLK
CSB-BP015164FLK
CSB-MP015164FLK
CSB-EP015164FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Ribonuclease 3 (rnc) CSB-YP019809FLK
CSB-EP019809FLK
CSB-BP019809FLK
CSB-MP019809FLK
CSB-EP019809FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis Phosphoserine aminotransferase (serC) CSB-YP018838FNR
CSB-EP018838FNR
CSB-BP018838FNR
CSB-MP018838FNR
CSB-EP018838FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri ATP synthase subunit delta (atpH) CSB-YP002355DUQ
CSB-EP002355DUQ
CSB-BP002355DUQ
CSB-MP002355DUQ
CSB-EP002355DUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033DUQ
CSB-EP018033DUQ
CSB-BP018033DUQ
CSB-MP018033DUQ
CSB-EP018033DUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DUQ
CSB-EP009582DUQ
CSB-BP009582DUQ
CSB-MP009582DUQ
CSB-EP009582DUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrococcus caseolyticus 6-phosphofructokinase (pfkA) CSB-YP017822MPA
CSB-EP017822MPA
CSB-BP017822MPA
CSB-MP017822MPA
CSB-EP017822MPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Elongation factor G (fusA), partial CSB-YP009373FMY
CSB-EP009373FMY
CSB-BP009373FMY
CSB-MP009373FMY
CSB-EP009373FMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae ATP synthase subunit delta (atpH) CSB-YP002355FMY
CSB-EP002355FMY
CSB-BP002355FMY
CSB-MP002355FMY
CSB-EP002355FMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146FMY
CSB-EP019146FMY
CSB-BP019146FMY
CSB-MP019146FMY
CSB-EP019146FMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium leprae ATP synthase subunit alpha (atpA), partial CSB-YP002344MVO
CSB-EP002344MVO
CSB-BP002344MVO
CSB-MP002344MVO
CSB-EP002344MVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Glycine cleavage system H protein (gcvH) CSB-YP009335FLK
CSB-EP009335FLK
CSB-BP009335FLK
CSB-MP009335FLK
CSB-EP009335FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Peptide chain release factor 1 (prfA) CSB-YP015197FLK
CSB-EP015197FLK
CSB-BP015197FLK
CSB-MP015197FLK
CSB-EP015197FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus ATP synthase subunit a (atpB), partial CSB-YP015070FLK1
CSB-EP015070FLK1
CSB-BP015070FLK1
CSB-MP015070FLK1
CSB-EP015070FLK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Porphobilinogen deaminase (hemC) CSB-YP010524FLK
CSB-EP010524FLK
CSB-BP010524FLK
CSB-MP010524FLK
CSB-EP010524FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus tRNA dimethylallyltransferase (miaA) CSB-YP024529FLK
CSB-EP024529FLK
CSB-BP024529FLK
CSB-MP024529FLK
CSB-EP024529FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>