Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Teredinibacter turnerae ATP synthase subunit a (atpB), partial CSB-YP015070TLR1
CSB-EP015070TLR1
CSB-BP015070TLR1
CSB-MP015070TLR1
CSB-EP015070TLR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Teredinibacter turnerae Protoheme IX farnesyltransferase (cyoE), partial CSB-YP005824TLR
CSB-EP005824TLR
CSB-BP005824TLR
CSB-MP005824TLR
CSB-EP005824TLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Teredinibacter turnerae Glycine cleavage system H protein (gcvH) CSB-YP009335TLR
CSB-EP009335TLR
CSB-BP009335TLR
CSB-MP009335TLR
CSB-EP009335TLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beutenbergia cavernae Elongation factor G (fusA), partial CSB-YP009373BST
CSB-EP009373BST
CSB-BP009373BST
CSB-MP009373BST
CSB-EP009373BST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrococcus luteus Porphobilinogen deaminase (hemC) CSB-YP010524MTP
CSB-EP010524MTP
CSB-BP010524MTP
CSB-MP010524MTP
CSB-EP010524MTP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kosmotoga olearia Serine hydroxymethyltransferase (glyA) CSB-YP021274KBR
CSB-EP021274KBR
CSB-BP021274KBR
CSB-MP021274KBR
CSB-EP021274KBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variovorax paradoxus Ribonuclease 3 (rnc) CSB-YP019809VEM
CSB-EP019809VEM
CSB-BP019809VEM
CSB-MP019809VEM
CSB-EP019809VEM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus sp. S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658GFJ
CSB-EP001658GFJ
CSB-BP001658GFJ
CSB-MP001658GFJ
CSB-EP001658GFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus sp. Cardiolipin synthase (cls), partial CSB-YP005985GFJ1
CSB-EP005985GFJ1
CSB-BP005985GFJ1
CSB-MP005985GFJ1
CSB-EP005985GFJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus sp. Thymidine kinase (tdk) CSB-YP023577GFJ
CSB-EP023577GFJ
CSB-BP023577GFJ
CSB-MP023577GFJ
CSB-EP023577GFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea thermotolerans Enolase-phosphatase E1 (UTR4) CSB-YP007674LMB
CSB-EP007674LMB
CSB-BP007674LMB
CSB-MP007674LMB
CSB-EP007674LMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Structure-specific endonuclease subunit SLX4 (SLX4), partial CSB-YP002843DOP
CSB-EP002843DOP
CSB-BP002843DOP
CSB-MP002843DOP
CSB-EP002843DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Flap endonuclease 1 (FEN1) CSB-YP008585DOP
CSB-EP008585DOP
CSB-BP008585DOP
CSB-MP008585DOP
CSB-EP008585DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Structure-specific endonuclease subunit SLX4 (SLX4), partial CSB-YP002843AZD
CSB-EP002843AZD
CSB-BP002843AZD
CSB-MP002843AZD
CSB-EP002843AZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Methylthioribose-1-phosphate isomerase (MRI1) CSB-YP014799AZE
CSB-EP014799AZE
CSB-BP014799AZE
CSB-MP014799AZE
CSB-EP014799AZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida tropicalis Methionine aminopeptidase 2 (MAP2) CSB-YP013716CZM
CSB-EP013716CZM
CSB-BP013716CZM
CSB-MP013716CZM
CSB-EP013716CZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus sibiricus Elongation factor 2 (fusA), partial CSB-YP007434TMT
CSB-EP007434TMT
CSB-BP007434TMT
CSB-MP007434TMT
CSB-EP007434TMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio salexigens Elongation factor Tu (tuf) CSB-YP025342DJQ
CSB-EP025342DJQ
CSB-BP025342DJQ
CSB-MP025342DJQ
CSB-EP025342DJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Glycine cleavage system H protein (gcvH) CSB-YP009335ESQ
CSB-EP009335ESQ
CSB-BP009335ESQ
CSB-MP009335ESQ
CSB-EP009335ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799ESQ
CSB-EP014799ESQ
CSB-BP014799ESQ
CSB-MP014799ESQ
CSB-EP014799ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>