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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida Malate dehydrogenase (mdh) CSB-YP013623FGA
CSB-EP013623FGA
CSB-BP013623FGA
CSB-MP013623FGA
CSB-EP013623FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida tRNA dimethylallyltransferase (miaA) CSB-YP024529FGA
CSB-EP024529FGA
CSB-BP024529FGA
CSB-MP024529FGA
CSB-EP024529FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Triosephosphate isomerase (tpiA) CSB-YP024102FGA
CSB-EP024102FGA
CSB-BP024102FGA
CSB-MP024102FGA
CSB-EP024102FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus Thymidylate kinase (tmk) CSB-YP007226TMI
CSB-EP007226TMI
CSB-BP007226TMI
CSB-MP007226TMI
CSB-EP007226TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter pseudethanolicus 6-phosphofructokinase (pfkA) CSB-YP017822TMI
CSB-EP017822TMI
CSB-BP017822TMI
CSB-MP017822TMI
CSB-EP017822TMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter sp. Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146TMJ
CSB-EP019146TMJ
CSB-BP019146TMJ
CSB-MP019146TMJ
CSB-EP019146TMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Choline transporter-like protein 5 (slc44a5), partial CSB-YP021652XBF
CSB-EP021652XBF
CSB-BP021652XBF
CSB-MP021652XBF
CSB-EP021652XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa ATP synthase subunit b (atpF), partial CSB-YP002358MTQ1
CSB-EP002358MTQ1
CSB-BP002358MTQ1
CSB-MP002358MTQ1
CSB-EP002358MTQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa Translation initiation factor IF-2 (infB), partial CSB-YP015164MTQ
CSB-EP015164MTQ
CSB-BP015164MTQ
CSB-MP015164MTQ
CSB-EP015164MTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa Serine hydroxymethyltransferase (glyA) CSB-YP021274MTQ
CSB-EP021274MTQ
CSB-BP021274MTQ
CSB-MP021274MTQ
CSB-EP021274MTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microcystis aeruginosa Ribosome-recycling factor (frr) CSB-YP014941MTQ
CSB-EP014941MTQ
CSB-BP014941MTQ
CSB-MP014941MTQ
CSB-EP014941MTQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes aegypti NADH-ubiquinone oxidoreductase chain 2 (mt:ND2), partial CSB-YP015077AXQ
CSB-EP015077AXQ
CSB-BP015077AXQ
CSB-MP015077AXQ
CSB-EP015077AXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laccaria bicolor Methylthioribose-1-phosphate isomerase (MRI1) CSB-YP014799LLZ
CSB-EP014799LLZ
CSB-BP014799LLZ
CSB-MP014799LLZ
CSB-EP014799LLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio anubis Proteasome assembly chaperone 1 (PSMG1) CSB-YP018921ERI
CSB-EP018921ERI
CSB-BP018921ERI
CSB-MP018921ERI
CSB-EP018921ERI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix jacchus Serpin B10 (SERPINB10) CSB-YP021066CYL
CSB-EP021066CYL
CSB-BP021066CYL
CSB-MP021066CYL
CSB-EP021066CYL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Malate dehydrogenase (mdh) CSB-YP013623BWU
CSB-EP013623BWU
CSB-BP013623BWU
CSB-MP013623BWU
CSB-EP013623BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Elongation factor G (fusA), partial CSB-YP009373BWU
CSB-EP009373BWU
CSB-BP009373BWU
CSB-MP009373BWU
CSB-EP009373BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824AVV
CSB-EP005824AVV
CSB-BP005824AVV
CSB-MP005824AVV
CSB-EP005824AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia rickettsii ATP synthase subunit alpha (atpA), partial CSB-YP002344RNB
CSB-EP002344RNB
CSB-BP002344RNB
CSB-MP002344RNB
CSB-EP002344RNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 3 N-acetylneuraminate lyase (nanA) CSB-YP015994AXH
CSB-EP015994AXH
CSB-BP015994AXH
CSB-MP015994AXH
CSB-EP015994AXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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