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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Porcine respiratory coronavirus Non-structural protein 7 (7), partial CSB-YP300228PQD
CSB-EP300228PQD
CSB-BP300228PQD
CSB-MP300228PQD
CSB-EP300228PQD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis delta virus genotype II Small delta antigen CSB-YP300229HFW
CSB-EP300229HFW
CSB-BP300229HFW
CSB-MP300229HFW
CSB-EP300229HFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T6 Translation repressor protein (regA) CSB-YP300230EEA
CSB-EP300230EEA
CSB-BP300230EEA
CSB-MP300230EEA
CSB-EP300230EEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP300231ANY
CSB-EP300231ANY
CSB-BP300231ANY
CSB-MP300231ANY
CSB-EP300231ANY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP300232CKQ
CSB-EP300232CKQ
CSB-BP300232CKQ
CSB-MP300232CKQ
CSB-EP300232CKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum marinum 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP300233HHX
CSB-EP300233HHX
CSB-BP300233HHX
CSB-MP300233HHX
CSB-EP300233HHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Setaria viridis 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP300234SFR
CSB-EP300234SFR
CSB-BP300234SFR
CSB-MP300234SFR
CSB-EP300234SFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP300235NHE
CSB-EP300235NHE
CSB-BP300235NHE
CSB-MP300235NHE
CSB-EP300235NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cercidiphyllum japonicum 30S ribosomal protein S7, chloroplastic (rps7) CSB-YP300236CGQ
CSB-EP300236CGQ
CSB-BP300236CGQ
CSB-MP300236CGQ
CSB-EP300236CGQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein T (psbT), partial CSB-YP300237LQQ
CSB-EP300237LQQ
CSB-BP300237LQQ
CSB-MP300237LQQ
CSB-EP300237LQQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Photosystem II reaction center protein T (psbT), partial CSB-YP300238TQN
CSB-EP300238TQN
CSB-BP300238TQN
CSB-MP300238TQN
CSB-EP300238TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyra umbilicalis Acetolactate synthase large subunit (ilvB), partial CSB-YP300239PQL
CSB-EP300239PQL
CSB-BP300239PQL
CSB-MP300239PQL
CSB-EP300239PQL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atropa belladonna Photosystem II reaction center protein J (psbJ), partial CSB-YP300240AQX
CSB-EP300240AQX
CSB-BP300240AQX
CSB-MP300240AQX
CSB-EP300240AQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat (tat) CSB-YP300241HKJ
CSB-EP300241HKJ
CSB-BP300241HKJ
CSB-MP300241HKJ
CSB-EP300241HKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine coronavirus Membrane protein (M), partial CSB-YP300242BJK1
CSB-EP300242BJK1
CSB-BP300242BJK1
CSB-MP300242BJK1
CSB-EP300242BJK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Woodchuck hepatitis B virus Capsid protein (C) CSB-YP300243WAW
CSB-EP300243WAW
CSB-BP300243WAW
CSB-MP300243WAW
CSB-EP300243WAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Rev (rev) CSB-YP300244HKQ
CSB-EP300244HKQ
CSB-BP300244HKQ
CSB-MP300244HKQ
CSB-EP300244HKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Vpr (vpr) CSB-YP300245HKQ
CSB-EP300245HKQ
CSB-BP300245HKQ
CSB-MP300245HKQ
CSB-EP300245HKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydrogenase-2 small chain (hybO) CSB-YP300246EGX
CSB-EP300246EGX
CSB-BP300246EGX
CSB-MP300246EGX
CSB-EP300246EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus bullatus Delta-conotoxin-like BVIA CSB-YP300247DVT
CSB-EP300247DVT
CSB-BP300247DVT
CSB-MP300247DVT
CSB-EP300247DVT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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