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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L11 (rplK) CSB-YP300495MLW
CSB-EP300495MLW
CSB-BP300495MLW
CSB-MP300495MLW
CSB-EP300495MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae UvrABC system protein B (uvrB), partial CSB-YP300496MLW
CSB-EP300496MLW
CSB-BP300496MLW
CSB-MP300496MLW
CSB-EP300496MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae UPF0134 protein MPN_104 (MPN_104) CSB-YP300497MLW
CSB-EP300497MLW
CSB-BP300497MLW
CSB-MP300497MLW
CSB-EP300497MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_205 (MPN_205) CSB-YP300498MLW
CSB-EP300498MLW
CSB-BP300498MLW
CSB-MP300498MLW
CSB-EP300498MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L3 (rplC) CSB-YP300499MLW
CSB-EP300499MLW
CSB-BP300499MLW
CSB-MP300499MLW
CSB-EP300499MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG148 homolog (MPN_161) CSB-YP300500MLW
CSB-EP300500MLW
CSB-BP300500MLW
CSB-MP300500MLW
CSB-EP300500MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Ribosome-binding factor A (rbfA) CSB-YP300501MLW
CSB-EP300501MLW
CSB-BP300501MLW
CSB-MP300501MLW
CSB-EP300501MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_096 (MPN_096), partial CSB-YP300502MLW1
CSB-EP300502MLW1
CSB-BP300502MLW1
CSB-MP300502MLW1
CSB-EP300502MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative type-1 restriction enzyme specificity protein MPN_089 (MPN_089) CSB-YP300503MLW
CSB-EP300503MLW
CSB-BP300503MLW
CSB-MP300503MLW
CSB-EP300503MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YafX (yafX) CSB-YP300504ENV
CSB-EP300504ENV
CSB-BP300504ENV
CSB-MP300504ENV
CSB-EP300504ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized symporter yagG (yagG), partial CSB-YP300505ENV
CSB-EP300505ENV
CSB-BP300505ENV
CSB-MP300505ENV
CSB-EP300505ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized membrane protein yahN (yahN), partial CSB-YP300506ENV1
CSB-EP300506ENV1
CSB-BP300506ENV1
CSB-MP300506ENV1
CSB-EP300506ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative Rz endopeptidase from lambdoid prophage DLP12 (rzpD) CSB-YP300507ENV
CSB-EP300507ENV
CSB-BP300507ENV
CSB-MP300507ENV
CSB-EP300507ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Dipeptide permease D (dtpD), partial CSB-YP300508ENV
CSB-EP300508ENV
CSB-BP300508ENV
CSB-MP300508ENV
CSB-EP300508ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Zinc transporter zitB (zitB), partial CSB-YP300509ENV1
CSB-EP300509ENV1
CSB-BP300509ENV1
CSB-MP300509ENV1
CSB-EP300509ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli PKHD-type hydroxylase ybiX (ybiX) CSB-YP300510ENV
CSB-EP300510ENV
CSB-BP300510ENV
CSB-MP300510ENV
CSB-EP300510ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybiU (ybiU) CSB-YP300511ENV
CSB-EP300511ENV
CSB-BP300511ENV
CSB-MP300511ENV
CSB-EP300511ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glutathione transport system permease protein gsiD (gsiD), partial CSB-YP300512ENV1
CSB-EP300512ENV1
CSB-BP300512ENV1
CSB-MP300512ENV1
CSB-EP300512ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ybjK (ybjK) CSB-YP300513ENV
CSB-EP300513ENV
CSB-BP300513ENV
CSB-MP300513ENV
CSB-EP300513ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydroxylamine reductase (hcp) CSB-YP300514ENV
CSB-EP300514ENV
CSB-BP300514ENV
CSB-MP300514ENV
CSB-EP300514ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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