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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Uncharacterized protein sll0720 (sll0720) CSB-YP300414SSQ
CSB-EP300414SSQ
CSB-BP300414SSQ
CSB-MP300414SSQ
CSB-EP300414SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable phosphoketolase (slr0453), partial CSB-YP300415SSQ
CSB-EP300415SSQ
CSB-BP300415SSQ
CSB-MP300415SSQ
CSB-EP300415SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cytochrome b6-f complex iron-sulfur subunit 1 (petC1), partial CSB-YP300416SSQ1
CSB-EP300416SSQ1
CSB-BP300416SSQ1
CSB-MP300416SSQ1
CSB-EP300416SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0426 protein ssl0294 (ssl0294) CSB-YP300417SSQ
CSB-EP300417SSQ
CSB-BP300417SSQ
CSB-MP300417SSQ
CSB-EP300417SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cytochrome b6-f complex subunit 7 (petM), partial CSB-YP300418SSQ
CSB-EP300418SSQ
CSB-BP300418SSQ
CSB-MP300418SSQ
CSB-EP300418SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell invasion protein sipA (sipA), partial CSB-YP300419SWW
CSB-EP300419SWW
CSB-BP300419SWW
CSB-MP300419SWW
CSB-EP300419SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Probable secretion system apparatus ATP synthase SsaN (ssaN) CSB-YP300420SXB
CSB-EP300420SXB
CSB-BP300420SXB
CSB-MP300420SXB
CSB-EP300420SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus V-type ATP synthase subunit C (atpC) CSB-YP300421TNT
CSB-EP300421TNT
CSB-BP300421TNT
CSB-MP300421TNT
CSB-EP300421TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima DNA mismatch repair protein mutL (mutL), partial CSB-YP300422TNJ
CSB-EP300422TNJ
CSB-BP300422TNJ
CSB-MP300422TNJ
CSB-EP300422TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Enolase (eno) CSB-YP300423TPR
CSB-EP300423TPR
CSB-BP300423TPR
CSB-MP300423TPR
CSB-EP300423TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lon protease (lon), partial CSB-YP300424VFE
CSB-EP300424VFE
CSB-BP300424VFE
CSB-MP300424VFE
CSB-EP300424VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized ATP-dependent helicase MG140 homolog (MPN_153), partial CSB-YP300425MLW
CSB-EP300425MLW
CSB-BP300425MLW
CSB-MP300425MLW
CSB-EP300425MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG061 homolog 2 (MPN_077), partial CSB-YP300426MLW
CSB-EP300426MLW
CSB-BP300426MLW
CSB-MP300426MLW
CSB-EP300426MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG055.2 homolog (MPN_070), partial CSB-YP300427MLW1
CSB-EP300427MLW1
CSB-BP300427MLW1
CSB-MP300427MLW1
CSB-EP300427MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Signal recognition particle protein (ffh) CSB-YP300428MLW
CSB-EP300428MLW
CSB-BP300428MLW
CSB-MP300428MLW
CSB-EP300428MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Phosphocarrier protein HPr (ptsH) CSB-YP300429MLW
CSB-EP300429MLW
CSB-BP300429MLW
CSB-MP300429MLW
CSB-EP300429MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG037 homolog (MPN_047) CSB-YP300430MLW
CSB-EP300430MLW
CSB-BP300430MLW
CSB-MP300430MLW
CSB-EP300430MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_040 (MPN_040) CSB-YP300431MLW
CSB-EP300431MLW
CSB-BP300431MLW
CSB-MP300431MLW
CSB-EP300431MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae DNA polymerase III polC-type (polC), partial CSB-YP300432MLW
CSB-EP300432MLW
CSB-BP300432MLW
CSB-MP300432MLW
CSB-EP300432MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_027 (MPN_027) CSB-YP300433MLW
CSB-EP300433MLW
CSB-BP300433MLW
CSB-MP300433MLW
CSB-EP300433MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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