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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Ethanolamine utilization protein eutJ (eutJ) CSB-YP301224ENV
CSB-EP301224ENV
CSB-BP301224ENV
CSB-MP301224ENV
CSB-EP301224ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized fimbrial-like protein ydeR (ydeR) CSB-YP301225ENV
CSB-EP301225ENV
CSB-BP301225ENV
CSB-MP301225ENV
CSB-EP301225ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable D,D-dipeptide transport system permease protein ddpB (ddpB), partial CSB-YP301226ENV1
CSB-EP301226ENV1
CSB-BP301226ENV1
CSB-MP301226ENV1
CSB-EP301226ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized sulfatase ydeN (ydeN) CSB-YP301227ENV
CSB-EP301227ENV
CSB-BP301227ENV
CSB-MP301227ENV
CSB-EP301227ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ycjZ (ycjZ) CSB-YP301228ENV
CSB-EP301228ENV
CSB-BP301228ENV
CSB-MP301228ENV
CSB-EP301228ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli p-aminobenzoyl-glutamate hydrolase subunit A (abgA) CSB-YP301229ENV
CSB-EP301229ENV
CSB-BP301229ENV
CSB-MP301229ENV
CSB-EP301229ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized ferredoxin-like protein ydhX (ydhX) CSB-YP301230ENV
CSB-EP301230ENV
CSB-BP301230ENV
CSB-MP301230ENV
CSB-EP301230ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybbN (ybbN) CSB-YP301231ENV
CSB-BP301231ENV
CSB-MP301231ENV
CSB-EP301231ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein phsC homolog (ydhU), partial CSB-YP301232ENV1
CSB-EP301232ENV1
CSB-BP301232ENV1
CSB-MP301232ENV1
CSB-EP301232ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybeU (ybeU) CSB-YP301233ENV
CSB-EP301233ENV
CSB-BP301233ENV
CSB-MP301233ENV
CSB-EP301233ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ybcY (ybcY) CSB-YP301234ENV
CSB-EP301234ENV
CSB-BP301234ENV
CSB-MP301234ENV
CSB-EP301234ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein hokB (hokB), partial CSB-YP301235ENV
CSB-EP301235ENV
CSB-BP301235ENV
CSB-MP301235ENV
CSB-EP301235ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized ABC transporter permease ybbP (ybbP), partial CSB-YP301236ENV
CSB-EP301236ENV
CSB-BP301236ENV
CSB-MP301236ENV
CSB-EP301236ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli FeS cluster assembly protein sufB (sufB) CSB-YP301237ENV
CSB-EP301237ENV
CSB-BP301237ENV
CSB-MP301237ENV
CSB-EP301237ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized GST-like protein yfcF (yfcF) CSB-YP301238ENV
CSB-EP301238ENV
CSB-BP301238ENV
CSB-MP301238ENV
CSB-EP301238ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ureidoglycolate dehydrogenase (allD) CSB-YP301239ENV
CSB-EP301239ENV
CSB-BP301239ENV
CSB-MP301239ENV
CSB-EP301239ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Mhp operon transcriptional activator (mhpR) CSB-YP301240ENV
CSB-EP301240ENV
CSB-BP301240ENV
CSB-MP301240ENV
CSB-EP301240ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybcV (ybcV) CSB-YP301241ENV
CSB-EP301241ENV
CSB-BP301241ENV
CSB-MP301241ENV
CSB-EP301241ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein flxA (flxA) CSB-YP301242ENV
CSB-EP301242ENV
CSB-BP301242ENV
CSB-MP301242ENV
CSB-EP301242ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized fimbrial chaperone yqiH (yqiH) CSB-YP301243ENV
CSB-EP301243ENV
CSB-BP301243ENV
CSB-MP301243ENV
CSB-EP301243ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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