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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma pneumoniae Ribonucleoside-diphosphate reductase subunit alpha (nrdE), partial CSB-YP301265MLW
CSB-EP301265MLW
CSB-BP301265MLW
CSB-MP301265MLW
CSB-EP301265MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Peptidyl-tRNA hydrolase (pth) CSB-YP301266MLW
CSB-EP301266MLW
CSB-BP301266MLW
CSB-MP301266MLW
CSB-EP301266MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yfeS (yfeS) CSB-YP301267ENV
CSB-EP301267ENV
CSB-BP301267ENV
CSB-MP301267ENV
CSB-EP301267ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Protein ARG5,6, mitochondrial (ARG5,6), partial CSB-YP301268CZF
CSB-EP301268CZF
CSB-BP301268CZF
CSB-MP301268CZF
CSB-EP301268CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Multidrug resistance protein CDR2 (CDR2), partial CSB-YP301269CZD
CSB-EP301269CZD
CSB-BP301269CZD
CSB-MP301269CZD
CSB-EP301269CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans pH-response regulator protein palF/RIM8 (palF), partial CSB-YP301270EKF
CSB-EP301270EKF
CSB-BP301270EKF
CSB-MP301270EKF
CSB-EP301270EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neocallimastix frontalis Malic enzyme, hydrogenosomal, partial CSB-YP301271NBQ
CSB-EP301271NBQ
CSB-BP301271NBQ
CSB-MP301271NBQ
CSB-EP301271NBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phanerochaete chrysosporium Manganese peroxidase H3 CSB-YP301272EUK
CSB-EP301272EUK
CSB-BP301272EUK
CSB-MP301272EUK
CSB-EP301272EUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Chitin synthase D (chsD), partial CSB-YP301273NGS1
CSB-EP301273NGS1
CSB-BP301273NGS1
CSB-MP301273NGS1
CSB-EP301273NGS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Vacuolar transporter chaperone 4 (vtc4), partial CSB-YP301274SXV1
CSB-EP301274SXV1
CSB-BP301274SXV1
CSB-MP301274SXV1
CSB-EP301274SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Acetyl-CoA carboxylase (cut6), partial CSB-YP301275SXV
CSB-EP301275SXV
CSB-BP301275SXV
CSB-MP301275SXV
CSB-EP301275SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative ribosomal RNA methyltransferase C2G2.15c (SPBC2G2.15c) CSB-YP301276SXV
CSB-EP301276SXV
CSB-BP301276SXV
CSB-MP301276SXV
CSB-EP301276SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Glyceraldehyde-3-phosphate dehydrogenase 1 (tdh1) CSB-YP301277SXV
CSB-EP301277SXV
CSB-BP301277SXV
CSB-MP301277SXV
CSB-EP301277SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Telomere length regulator taz1 (taz1), partial CSB-YP301278SXV
CSB-EP301278SXV
CSB-BP301278SXV
CSB-MP301278SXV
CSB-EP301278SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans pH-response regulator protein palA/RIM20 (palA), partial CSB-YP301279EKF
CSB-EP301279EKF
CSB-BP301279EKF
CSB-MP301279EKF
CSB-EP301279EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geotrichum fermentans Lipase 1 (LIP1), partial CSB-YP301280GBQ
CSB-EP301280GBQ
CSB-BP301280GBQ
CSB-MP301280GBQ
CSB-EP301280GBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capreolus capreolus Brain ribonuclease (BRN) CSB-YP301282DQB
CSB-EP301282DQB
CSB-BP301282DQB
CSB-MP301282DQB
CSB-EP301282DQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fundulus heteroclitus Type II iodothyronine deiodinase (dio2), partial CSB-YP301283FDK1
CSB-EP301283FDK1
CSB-BP301283FDK1
CSB-MP301283FDK1
CSB-EP301283FDK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken T-box-containing protein TBX6L (TBX6L) CSB-YP301284CH
CSB-EP301284CH
CSB-BP301284CH
CSB-MP301284CH
CSB-EP301284CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja atra Cytotoxin 1c CSB-YP301285NFG
CSB-EP301285NFG
CSB-BP301285NFG
CSB-MP301285NFG
CSB-EP301285NFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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