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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sheep Metallothionein-2 (MT2) CSB-YP303037SH
CSB-EP303037SH
CSB-BP303037SH
CSB-MP303037SH
CSB-EP303037SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-directed RNA polymerase 7 kDa subunit (RPO7) CSB-YP303038VAA
CSB-EP303038VAA
CSB-BP303038VAA
CSB-MP303038VAA
CSB-EP303038VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 7 Triplex capsid protein U56 (U56) CSB-YP303039HKC
CSB-EP303039HKC
CSB-BP303039HKC
CSB-MP303039HKC
CSB-EP303039HKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Envelope glycoprotein H (gH), partial CSB-YP303040EFL
CSB-EP303040EFL
CSB-BP303040EFL
CSB-MP303040EFL
CSB-EP303040EFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Packaging protein 300, partial CSB-YP303041EFI
CSB-EP303041EFI
CSB-BP303041EFI
CSB-MP303041EFI
CSB-EP303041EFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epstein-Barr virus Uncharacterized protein BLLF2 (BLLF2) CSB-YP303042EEZ
CSB-EP303042EEZ
CSB-BP303042EEZ
CSB-MP303042EEZ
CSB-EP303042EEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquila audax Ovomucoid CSB-YP303043ANA
CSB-EP303043ANA
CSB-BP303043ANA
CSB-MP303043ANA
CSB-EP303043ANA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Grus carunculatus Ovomucoid CSB-YP303044GDO
CSB-EP303044GDO
CSB-BP303044GDO
CSB-MP303044GDO
CSB-EP303044GDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callipepla gambelii Ovomucoid CSB-YP303045CAU
CSB-EP303045CAU
CSB-BP303045CAU
CSB-MP303045CAU
CSB-EP303045CAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astrotia stokesii Short neurotoxin 1 CSB-YP303046AQF
CSB-EP303046AQF
CSB-BP303046AQF
CSB-MP303046AQF
CSB-EP303046AQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti U1-theraphotoxin-Hh1f CSB-YP303047HUL
CSB-EP303047HUL
CSB-BP303047HUL
CSB-MP303047HUL
CSB-EP303047HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Scaffold protein D13 (VACWR118), partial CSB-YP303048VAI
CSB-EP303048VAI
CSB-BP303048VAI
CSB-MP303048VAI
CSB-EP303048VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phasianus colchicus colchicus Ovomucoid CSB-YP303049EUO
CSB-EP303049EUO
CSB-BP303049EUO
CSB-MP303049EUO
CSB-EP303049EUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 9.3 kDa protein (D ORF E) CSB-YP303050VAI
CSB-EP303050VAI
CSB-BP303050VAI
CSB-MP303050VAI
CSB-EP303050VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.2 kDa protein (D ORF H) CSB-YP303051VAA
CSB-EP303051VAA
CSB-BP303051VAA
CSB-MP303051VAA
CSB-EP303051VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Metallothionein CSB-YP303052CH
CSB-EP303052CH
CSB-BP303052CH
CSB-MP303052CH
CSB-EP303052CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salvelinus alpinus Metallothionein A (mta) CSB-YP303053SBW
CSB-EP303053SBW
CSB-BP303053SBW
CSB-MP303053SBW
CSB-EP303053SBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyprinus carpio Fish-egg lectin CSB-YP303054EQE
CSB-EP303054EQE
CSB-BP303054EQE
CSB-MP303054EQE
CSB-EP303054EQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus durissus durissus Disintegrin durissin CSB-YP303055CSW
CSB-EP303055CSW
CSB-BP303055CSW
CSB-MP303055CSW
CSB-EP303055CSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxoma virus DNA-directed RNA polymerase 21 kDa subunit (RPO22) CSB-YP303056MMT
CSB-EP303056MMT
CSB-BP303056MMT
CSB-MP303056MMT
CSB-EP303056MMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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